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Camellia (Camellia oleifera) is a unique woody edible oil plant in southern China , with oil palm , olive , coconut and called the world's four major woody edible oil plants . Camellia oleifera tissue culture technology , the pilot study , the main findings are as follows : 1 , Camellia tissue culture year in July , Camellia oleifera tissue culture explants acquisition period , the use of 1 -year-old Camellia oleifera Cutting new pumping buds as explants than 3 -year-old new pumping buds as explants physical more effective to improve the induction rate , at the same time reduce the contamination rate and browning rate . 2 , Camellia with bud stem segments after pretreatment with 75% alcohol rapid disinfection of 30s and the 0.1% Hgcl2 disinfection 6 min after inoculation in MS 6 - BA 2.0 mg / L NAA0.2 mg / L medium bud best callus induction effect . Different Camellia clones induced rate there are obvious differences in the three clones (' Cen soft ' , ' soft Cen 3' and ' Gui soft on the 11th ') induced 57 % , 67% and 33 %. In addition, the callus in MS 6-BA0.6 mg / L NAA2.0 mg / L proliferation following the proliferative effect on the generation of medium best . 3 , the following medium conditions most suitable for Camellia organization culture: MS medium medium distilled water configuration , sucrose 30 g / L, agar 0.51% , pH value of 5.8 . Take direct light train ( light intensity about 14001x illumination time of 12 h / d) after vaccination before dark for 7 days compared to the light culture processing , cultivating good effect after vaccination . 4 , Camellia embryogenic callus medium : MS 2,4- D2 .0 mg / L KT, 1.Omg / L , the induction rate reached 73% . Embryogenic callus , transferred to MS 6 - BA0.6 , mg / L NAA2.0 mg / L embryoid induction medium highest in the induction rate reached 73% . 5 , the Camellia sterile seedling, the best medium for : SH 6 - BA 2.0 mg / L NAA0.2 mg / L
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