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Analyse Genetic Relationship and Diversity of the Different Geographic Liriodendron by ISSR ZHAN Zhiyong

Author: ZhanZhiYong
Tutor: XuGangBiao
School: Central South University of Forestry Science and Technology
Course: Tree Genetics and Breeding
Keywords: Liriodendron ISSR Cluster analysis Genetic Diversity
CLC: S792.21
Type: Master's thesis
Year: 2010
Downloads: 59
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Abstract


Some have been lost, of Liriodendron genetic resources and scientific conservation and rational utilization of existing natural populations , we must first understand their intraspecific genetic diversity and genetic differentiation , germplasm resources protection and the genetic improvement of the species theory basis and foundation material . In this study, the use of ISSR Liriodendron of 20 different geographical provenance of genetic analysis results are as follows : . Liriodendron ISSR-PCR Reaction System : In this experiment , the CTAB method to extract the various samples plant genomic DNA and ISSR reaction process in a number of important parameters exploration and optimization , to establish a stable , suitable for the Liriodendron genetic diversity of ISSR-PCR reaction system . After optimization of the reaction system : 20 μL of the total system , containing 30 ng template DNA.0.3μmol · the random primers L -1 , 0.2 mmol · L- 1 dNTPs , 1.4 mmol.L - 1Mg2 , 0.4 U of Taq DNA polymerase . Optimal amplification conditions 1) initial denaturation : 94 ° C for 5 min ; 2 ) denaturation : 94 ° C , 1 min ) annealing :50- 56 ° C , 45 s , 4 ) an extension : 72 ° C , 2 min ; Total 45 cycles ; final extension at 72 ℃ 7 mmin . Liriodendron population genetic diversity analysis : Application of ISSR genetic diversity of the 20 geographical provenance of a total of 200 individuals . Eight primers screened from 70 primers . 8 were amplified with primers to the 70 sites , 43 of which are polymorphic , the total percentage of polymorphic loci (P) 61.43% . Nei's genetic diversity index (H) .1717 Shannon polymorphism information index ( I ) 0.2722 20 levels of genetic diversity of different provenances little difference . Groups polymorphism loci between 49.87% -79.32 % , Nei 's genetic diversity index 0.1262-0.1876 and 0.1915-0.6173 Shannon polymorphism information index . Coefficient of genetic differentiation among the 20 populations Gst was 0.6173 , indicating there is a certain degree of genetic differentiation between the groups . Liriodendron group clustering analysis : according to Nei's genetic distance UPGMA cluster analysis of the results , the five kinds of North American Liriodendron source clustered into one group ; Liriodendron can be clustered into three main groups , namely the southern region , the northern region and central regions.

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Broad-leaved trees > Liriodendron
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