Dissertation > Excellent graduate degree dissertation topics show

The Research on Camellia Oleifera Root Rot and Its PCR Rapid Detection Techolgoy

Author: HaoFang
Tutor: ZhouGuoYing
School: Central South University of Forestry Science and Technology
Course: Microbiology
Keywords: Camellia oleifera root rot pathogencity biological characterstics nested-PCR
CLC: S763.7
Type: Master's thesis
Year: 2009
Downloads: 43
Quote: 0
Read: Download Dissertation

Abstract


Camellia oleifera root rot was one kind of root rots which usually jeopardized Camellia oleifera.The root of Camellia oleifera harmed came to rot,which leaded to the death of the plant because of the unusual sorbation of nutiaton and water and brought serious danger to Camellia oleifera production. It has been one of limiting factors to Sustainable Development of Camellia oleifera.In this paper, Camellia oleifera root rot was identified and biological characteristics,nested PCR disease dianosis techolgoy were studied.The pathogens of Camellia oleifera root rot had been separated and identified.Collected 14 species plant culivars infected Camellia oleifera root rot and only one pathogen was isolated.The isolate was inoculated the healthy plant in two means.The results showed that the plant had the same symptom with the speciemen which had gathered from filed.Then isolated from the plant agina,the fungi were were the same pathogen which had isolated from specimen, the pathogenic fungi had strong pathogenicity. Morphological characteristics were expatiated and sequenced the strain of the rDNA internal transcribed spacer (ITS). The homologous strain in the Genbank were download, molecular phylogenetic tree was constructed. Based on morphological characteristics and molecular phylogenetic tree, it can be concluded that the pathogens is Fusarium moniliform.The pathogen’s characterization had been studied.It showed that the optimum temperature and pH for mycelial growth of the pathogen were 25℃and pH 5-7.Different lighting time had no significant effect on mycelium growth of Fusarium moniliform.Condia could not germinate if the relative humidity was lower than 90%,the geimation rate was the higest in the water drop.The lethal temperature was 60℃,10minutes, Starch is the best carbon nutrient,NH4Cl is the best nitrogen nutrient.Rapid and accurate detection of the pathogenic of Root Rot of Camellia oleifera diseased plant tissues was developed in the study. Based on differences in internal transcribed spacer(ITS) sequences of Fusarium., a pair of species-specific primers, G1/G2 was synthesized. The others species of Fusarium were used to test the specificity of the primers. G1/G2 amplified only a unique 400 bp band from CSUFT070109 strain. The detection sensitivity with G1/G2 was 1 pg of genomic DNA in 25μL recation solution. A nested PCR procedure using ITS1/ITS4 as the first-round primers and followed with G1/G2 increased detection sensitivity 10000-fold to 100 ag. The results suggested that the assay detected the pathogen more rapidly and accurately than the standard isolation methods.The PCR-based methods developed here could simplify both plant disease diagnosis and pathogen monitoring, as well as guiding plant disease management.In this study,we identified the pathogen causing Camellia oleifera root rot as Fusarium moniliform.providing information to protect and control Camellia oleifera correctly.this pathogen’s characterization was studied to make clear its surrounding and make proper programme how to control root rot efficiently. Besides,through the nested PCR dianosis techololigy studied we could also established one efficient methods for early disease dianosis,pest control,monitoring and forecasting of Camellia oleifera root rot

Related Dissertations

  1. Screening of Pathogenicity-Ralated Genes and Function Analysis of luxR Gene in Acidovorax Avenae Subsp. Citrulli,S436.5
  2. Shanghai a porcine Cryptosporidium parasite identification and Zunyi City Cryptosporidium infection in humans and animals Preliminary Investigation,S855.9
  3. The Effect of Zn on Co-culture System of Three Arbuscular Mycorrhizal Fungi and Transformed Roots of Astragalua Sinicus L.,S551.9
  4. Establishment of a Nested PCR Assay and Analysis of the Full Genome of HUMAN COSAVIRUS,S855.3
  5. The Interaction and Effect of Inoculation of AM Fungi, Phosphate-Solubilizing Bacteria and Rhizobium on Growth of Astragalus Sinicus L. at Different Phosphate Level,S154.3
  6. The pCMV DPOL gene cloning analysis and construction and application of this gene PCR - based detection methods,S852.65
  7. Multiplex PCR Method for children with respiratory tract infection characterized pedigree,R440
  8. The Early Diagnosis of HFRS with PCR-ELISA,R512.8
  9. Monitoring the Effect of Cadmium Oc Astragalus L. Inoculated with Abuscular Mycorrhizal Fungi,S541.3
  10. Detection and Complete Genome Sequencing of Porcine Circovirus Type 2 in Different Pig Herds,S852.65
  11. Comparative Analysis of Fluoresenes Quantitative-PCR and Reverse Transcription-PCR in Detecting HCV-RNA,R446.9
  12. The Developement of Rapid Detection and Drug Susceptibility Testing for the Bovine Mastitis Pathogens,S858.23
  13. Pregnant cows free fetal DNA in peripheral blood Detection and Identification of gender,S823
  14. The Detection of HCMV DNA in Patients with Atherosclerotic Ischemic Cerebrovascular Diseases,R743.3
  15. Investigation on the Key Transmission Routes of WSSV in the Culture of Fenneropenaeus Chinensis,S945
  16. Cloning of p80 Gene of Classical Swine Fever Virus and Expression in E.coli.,S852.65
  17. Detection of donor-derived chimeric cells in peripheral lymphocytes of renal allograft recipients and the pilot study between donor specific bone marrow transfusion and chimerism,R699.2
  18. Avian infectious bronchitis virus S1 gene RT- PCR and RT - nested PCR amplification and sequence analysis,S852.65
  19. Pandemic strain of rubella virus isolation and identification of its E1 region gene sequence analysis,S852.65
  20. Research for the Human Perripheral Blood Mitochondrial DNA4977bp Deletion and Ionizing Radiation,R144

CLC: > Agricultural Sciences > Forestry > Forest Protection > Forest Pest and Disease Control > All kinds of tree pests and diseases and their prevention
© 2012 www.DissertationTopic.Net  Mobile