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Dissection of Resistant Genes to Northern Corn Leaf Blight in CIMMYT Maize Cultivar Ent17 and Bioinformatics Analysis of Candidate Sequences between Two Flanking Markers Linked with Resistance Gene Ht1

Author: EYang
Tutor: LinFeng;WangYanBo
School: Shenyang Agricultural University
Course: Developmental Biology
Keywords: Corn Corn leaf blight Bioinformatics Candidate sequence
CLC: S435.11
Type: Master's thesis
Year: 2010
Downloads: 73
Quote: 0
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Abstract


Corn leaf blight is caused by turcica (Exserohilum turcicum) corn leaf diseases, seriously restricting China's corn production. Due to the the turcicum physiological races variation, resulting in the loss of the original disease-resistant maize varieties resistant. Corn turcica resistance gene is divided into a number of resistance and mass resistance. Fostering the aggregation of multi-resistant genetically modified varieties prevention and treatment of a large blotch is the most cost-effective methods. CIMMYT maize germplasm Ent17 blotch resistance is excellent, but it contains the resistance gene is still unclear. To this end, this study have been reported with the big leaf spot resistance gene closely linked markers around the SSR Markers and Field Identification of resistance, Ent17 contained large blotch resistance genes identified, and to construct a genetic map. At the same time, the experimental screening SSR primers for amplification primers selected to demonstrate the applicability of 27 maize varieties. When the multi-gene is present in the same species, gene interaction phenomenon, the phenotype is a multi-gene interactions reflect. Therefore, this experiment Ent17 resistance gene contained in big blotch resistance gene in heterozygous state resistance performance. Cloned gene is not confrontational gene. Spot disease resistance gene Htl, for example, the use of bioinformatics methods of molecular markers tightly linked with the corn leaf blight resistance gene HTL candidate sequences were analyzed within UMC22 and of positioning umc122 area. The main findings are as follows: 1.Ent17 containing the resistance gene Htl and HT2. 9-SSR markers and resistance genes Htl linkage relationship (genetic distance of 9.7 cM to 13.1 cM). 11 SSR markers and resistance genes Ht2 linkage relationship. The Htl the flanking markers umc1042 and bnlg198, and Ht2 flanking markers umc1777 umc1665 for assisted breeding, Htl and Ht2, aggregated into maize varieties. 2 corn leaf blight resistance gene HTL candidate sequences, articles bioinformatics methods closely linked molecular corn leaf blight resistance gene HTL mark the positioning area UMC22 and umcl22 candidate sequence analysis, which get 63 ORF sequence of 14 sequences encoding protein domains. 14 nucleotide acid sequence predicted amino acid sequence and the cloned 24 resistance gene encoding the amino acid sequence the Blast alignment and phylogenetic tree constructed. It was found that the the candidate sequence gpm565a plant resistance gene encodes a product of a highly conserved domain, and the similarity with the resistance gene Xal, kinship, presumably related to the resistance gene Htl. The other candidate sequence does not have a plant resistance gene encodes a product highly conserved domains or similarity, relationship far, can not be determined with the resistance gene Ht1. On the the candidate sequences gpm565a secondary structure and three-dimensional structural analysis, found that the presence of a large number of specific features of structural elements that make up proteins random coil, to speculate gpm565a may be part of the Ht1 functional domains.

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Cereal crop pests and diseases > Rice pests and diseases
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