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Growth of Soil Algae on Different Substrates-An Ecosystem Approach to Soil Crusts

Author: ZhangXin
Tutor: WangHaiZeng;Prof. Dr. Hartmut Koehler
School: Ocean University of China
Course: Marine Chemistry
Keywords: Combat Desertification Cyanobacteria Green algae Soil Crusts Biochar Aquasorb
CLC: S154
Type: Master's thesis
Year: 2010
Downloads: 79
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Abstract


This paper studies the filamentous green algae and cyanobacteria based natural wild algae growth in ordinary soil and desert soil cleaned. Add 10% and 30% clay, respectively, in the two types of soil, 1% insurance agent, 10% and 30% bio-carbon, 10% and 30% of organic fertilizer as well as the ratio of clay: the biological carbon: organic fertilizer: aquasorb = 10:10:10:1 compound fertilizer, more algae growth. The first test of the performance of the soil before planting soil algae: soil water retention and soil pH. The analysis showed that the water-holding capacity of the soil of the desert, using 30% of organic fertilizer, can greatly improve the water retention properties of the soil. Filamentous green algae used in the experiment from the University of Goettingen, Germany EPSAG experimental institutions, the first in a Petri dish culture of green algae, use BBM V culture medium, wait until the breeding to a certain number, transferred into culture flasks train large numbers of to use BBM SE culture medium. Incubation temperature 25 ° C, 16 hours of light and 8 hours of darkness, the light intensity of 5000 Lux. Green algae in culture flasks month after transplantation into the soil. Experiment wild algae collected in the the Bremen University RevitalTec experimental fields, wild algae cyanobacteria, there is a small amount of filamentous green algae and unicellular algae. Buoyancy device will be collected in the wild algae enriched by the line, and then planted to different soil. The two algae grown in a greenhouse for 3 months, watering once every three days, the last measurement of algal biomass, and soil crusts, pH. This experiment used the indirect counting method of measuring algal biomass: under a fluorescence microscope to measure the frequency of occurrence of algae in the screen, and thus indirectly calculated relative biomass of algae. The observation area of ??each slide 20, note the probabilities of occurrence of algae in each region on the screen. Data analysis using SPSS general linear model (General Linear Model). Results: green algae growth conditions generally better than the wild algae, algae grow in ordinary soil cleaning. Green algae, use of biological carbon and clay, can greatly improve its biomass. For wild algae use aquasorb most favorable to their growth. Before and after the growth of algae, two soil pH were significant changes. Algae growth, desert soil and cleaning ordinary soil pH between 7.3-7.4; algae growth, the desert soil pH about 6.7, cleaning ordinary soil pH around 6.4. In the the soil crusts analysis results show that the desert soil crust is better, add clay and biological carbon, can significantly increase the amount of soil crust. When you add organic fertilizers, soil crust is less than the control experiment, the surface of the soil is soft. Correlation (correlation) analysis using SPSS correlation of soil crusts, soil pH, and two species of algae biomass. The results showed: green algae biomass and soil crusts and experimental pH value, the biomass with the knot increase in the amount of skin and increases when the pH is 6.8-6.9 green algae biomass peak. Wild algae and soil crusts, pH had no significant correlation. The experimental results show that bio-carbon and clay is used as a soil additive can promote the growth of soil algae. So they have an important role in the prevention and control of desertification. Found in the experiment, the growth of filamentous green algae in the humid climate situation is better than cyanobacteria, but for both growth conditions in the desert ecosystem is not yet clear.

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