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Court drug residue analysis is an analysis of trace components in complex mixtures , it requires fine trace operation means high sensitivity and specificity . Because of the complexity of the components of biological samples , and the physical and chemical properties of the different varieties of drugs are quite different and therefore not able to cover all varieties of drug residue analysis of a multi-component . To this end , this paper studies the impact of various factors on the efficiency of the microwave-assisted solvent extraction , gel chromatography purification concentrated purifying effect spectrometry of biological samples in fats, cholesterol and other impurities , with a mixed solvent the method was extracted under the same conditions of different types of pesticides . First , this study using gel chromatography purification concentrated spectrometer , the establishment of a pesticide , sleeping pills , drug purification concentrated extracts of biological samples . Instruments by changing the impurity cancellation time , the collection time, low degree of vacuum , and other parameters laser , increase the recovery rate of the drug , reduce the content of impurities in the extract . Purify concentrated Act may be batch of drugs used in the actual case , the method is simple , reproducible , grease, pigments and other substances purification better meet the requirements of the toxicology analysis . Secondly, study the factors that affect the extraction efficiency of the extraction solution , extraction time , the equilibrium temperature conditions , the use of microwave -assisted solvent extraction the instrument and PTV-GC/MSMS technology simultaneous analysis of 23 kinds of organophosphorus pesticides in the blood , 23 pesticides recoveries between 63% -96.8 % precision in 1.76-11.1 % ; detection limit of 5-60 ng · mL - 1 ; This method is rapid , simple , small the good reproducibility blank interference batch for actual cases of drug toxicology screening , and time - to - test analysis . Finally , the paper examines the impact factors of the extraction time , extraction temperature urethane and blood the Adenophora toxins pesticides extraction rate , the establishment of microwave-assisted solvent extraction , gel chromatography purification concentrated spectrometry and gas chromatography mass spectrometry spectrometer combination of analytical methods , various types of pesticides recoveries between 68-91 % , the extract was set the volume to 2 mL , the detection limit of 0.001-0.47μg.mL -1 .
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