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The first part of the local injection of alendronate on orthodontic tooth movement in rats [Abstract] Objective To observe the local injection of alendronate on orthodontic tooth movement in rats and periodontal tissue. Methods 32 Wistar rats were divided into three experimental groups, 40g force to pull the left maxillary first molar mesial movement experiments were saline control group and low dose, medium dose, high dose, 0.02mmol / L, 0.1 mmol / L, 0.5 mmol / L of alendronate solution was injected into rats in each group maxillary first molar palatal side mucoperiosteal injection three days before the experiment began, once every three days, every time 50 μl. Respectively in 0,1,3,7,14,17,21 days modulo method to measure the amount of tooth movement in rats. 21 days take maxillary tissue blocks by fixed, decalcified, dehydrated, embedded slices, HE staining observed changes in the periodontal tissue, TRAP staining pressure zone number of osteoclasts, break cementum cell number, whether the comparison groups There are differences. ○ 1 in each experimental group rat first molar moving distance was significantly lower than the control group, and its inhibitory effect enhanced with the increased concentration of the drug. ○ experimental group pressure zone osteoclast cell number and the number of broken cementum cells was significantly lower than the control group. Conclusions local injection of different concentrations of alendronate for the inhibitory effect of tooth movement, can be applied clinically as an enhanced means of anchorage. The impact of the second part of the local injection of alendronate rat orthodontic tooth movement in periodontal tissue [Abstract] Objective To observe the local injection of alendronate periodontal tissues of rats during orthodontic tooth movement organized over time Histological changes. Methods 40 Wistar rats were randomly divided into control and experimental groups, Each group was randomly divided into group 0d, 3d, 7d, 14d, 21d group. 40g force led by the left maxillary first molar mesial movement experiments were saline and 0.1 mmol / L alendronate solution was injected into two groups of rats maxillary first molar palatal mucoperiosteal injection at 3 days before the experiment began, once every three days, each 50 μl. The groups were first 0d, 3d, 7d, 14d, 21d killed, TRAP staining and HE staining of tissue sections were counting the number of osteoclasts and the number of broken cementum cells and observe the changes of periodontal tissue morphology over time. Experimental group, the number of osteoclasts in 3d, 7d, 14d and 21d, significantly lower than the control group. Experimental group breaking cementum cells 7d, 14d and 21d, the number was significantly lower than the control group. Conclusions Topical application of alendronate to reduce the amount of tooth movement in rats by inhibiting the activity of osteoclasts, the inhibition of root resorption.
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