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Salsa (Suaeda salsa) EST database construction and gene sequence analysis

Author: ZhangLei
Tutor: ZhaoYanXiu
School: Shandong Normal University
Course: Developmental Biology
Keywords: stress-tolerant models EST cDNA library euhalophytic Suaeda salsa GenBank
CLC: Q943.2
Type: Master's thesis
Year: 2001
Downloads: 142
Quote: 0
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Abstract


Agricultural productivity is severely affected by soil salillity. Past effOrts toinlprove plant tolerallce to osmotic stI’ess have llacl linlited sLlccess owillg to tllegelletic colllplexity of stress responses. IiS’I’ (expressed seqtlencc tags) dataacculllulation f’l.onl stress-toIerant modeIs is a rclatively rapid alld cheaP wayfOr discovering new genes l.elated to osnlotic stresS. Sllaed’I sal3u, wllicIl calltolel.ate lligll saIinity, is a favorite eullal()l7Ilytic nlodel f()r salillity tolerantresearch. We disl)lay llere the collstructioll of a NaCl-treated cDNA library andsingle-pass sequencing of randonlly selected clolles. ILS"I. (t()tal l000, 773 ofwllich have similarity to known gelles) were generated alld submitted to db[iS’ltill GellBank. Analysed with so\ftwalles, nlany gelles relate(l to salinity stresstolerance and new genes were found in our dbFS*I’. It is believed that ourdbEST and the associated DNA materials will be a applicable soul’ce toscientists engaging in stress-tolerance sludy.

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CLC: > Biological Sciences > Botany > Plant Cell Genetics > Plant Genetic Engineering
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