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The Expression and Significance of WWOX, Bcl-2 in Human Thyroid Papillary Carcinoma

Author: CaoDengZuo
Tutor: ChenKuiSheng；ZhangHongXin
School: Zhengzhou University
Course: Pathology and Pathophysiology
Keywords: Papillary thyroid carcinoma Dual - tryptophan domains oxidoreductase B - cell lymphoma / leukemia 2 gene Immunohistochemistry In situ hybridization
CLC: R736.1
Type: Master's thesis
Year: 2010
Downloads: 29
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Abstract

Thyroid cancer is one of the most common malignant tumors of the head and neck, accounting for 1.3% of all malignant tumors, accounting for 0.4% of cancer deaths. It can occur at any age, but more common 40-50 years old, the male to female ratio of approximately 2:3. The incidence of thyroid cancer mechanism is not yet clear. With the rapid development of molecular biology techniques, from the level of the gene have a greater understanding of the pathogenesis of thyroid cancer. The double tryptophan oxidoreductase domain (WW domain-containing oxidoreductase, WWOX) gene is common fragile sites in chromosomes Bednarek et al shotgun gene sequencing technology to identify a new tumor suppressor gene in 2000, in the outside world under the influence of factors such as radiation, viruses, certain toxins, etc. under the action of the mutation or deletion of the gene fragments are likely to occur, if the variation or the missing part is just able to inhibit abnormal cell growth gene, then this variation and deletions may inhibit apoptosis and promote tumor occurrence and development. WWOX or by Tyr33 phosphorylation and activation, reduced apoptosis inhibitory sub-Bcl-2 and Bcl-xL raised apoptotic protein p53, thereby enhancing the cytotoxic effect of TNF, and can form complexes with p53 mediated Apoptosis by common death, thereby inhibiting the development of tumor cells. Bcl-2 gene, also known as B cell lymphoma / leukemia -2 (B-cell lymphoma/euke-2) gene play a biological role, it is widely inhibit apoptosis induced by a variety of stimuli, extend cell viability. Bcl-2 protein confrontation to mitochondrial rupture caused by ion loss cross, thereby blocking the release of cytochrome C, can directly inhibit the activation of Caspase. The gene expression the protein inhibit apoptosis in a variety of tissue cells and prolong cell life, called apoptosis suppressor gene. WWOX and Bcl-2 expression in cervical squamous cell carcinoma, leukemia, ovarian epithelial tumors, liver cancer cell lines have been reported in the literature, but the development of the relationship between the two with the incidence of thyroid cancer research has so far not been reported. In this study, the SP method of immunohistochemistry and in situ hybridization to detect normal thyroid tissue, thyroid adenoma and thyroid carcinoma WWOX expression in thyroid carcinomas with the expression of Bcl-2, and both correlation analysis, explore the WWOX and Bcl-2 in the development of thyroid cancer, and clinical significance to provide the theoretical basis for further study of the incidence of thyroid cancer mechanism. Materials and methods using the SP method of immunohistochemistry and in situ hybridization to detect 40 cases of normal thyroid tissue, the WWOX expression of Bcl-2, and both in 36 patients with thyroid adenoma and 60 cases of thyroid papillary carcinoma expression in papillary thyroid carcinoma tissue correlation analysis. 2. Statistical analysis: All data used SPSS10.0 statistical software for statistical analysis, the x2 test was used to compare the positive rate, correlation between the positive rate using spearman correlation analysis testing, inspection standards to, α = 0.05 significant test standards. Results 1. WWOX protein in normal thyroid tissue, thyroid adenoma, papillary thyroid cancer tissues, the positive expression rate was 95.00% (38/40), 63.89% (23/36) and 48.33% (29/60). WWOX protein expression was significantly lower than in papillary thyroid carcinoma thyroid adenoma and normal thyroid tissue, and the difference was statistically significant (P lt; 0.05). Normal thyroid tissue, thyroid adenoma and papillary thyroid carcinoma tissue of WWOX mRNA positive expression rate of 97.50% (39/40), 63.89% (23/36) and 50.00% (30/60). WWOX mRNA positive expression rate was significantly lower than in thyroid cancer thyroid adenoma and normal thyroid tissue, differences with statistical significance (P lt; 0.05) WWOX protein and mRNA expression in thyroid carcinomas with the sex of the patient, regardless of age (P gt; 0.05). Lymph node metastasis WWOX protein expression was lower than that found no obvious lymph node metastasis, and between the two, and the difference was statistically significant (P lt; 0.05). 4. Bcl-2 protein expression in normal thyroid tissue, thyroid adenoma and papillary thyroid carcinoma tissue rate were 32.50% (13/40), 50.00% (18/36) and 81.67% (49/60) . Bcl-2 protein expression was significantly higher than in papillary thyroid carcinoma thyroid adenoma and normal thyroid tissue, and the difference was statistically significant (P lt; 0.05). 5. Bcl-2 mRNA in normal thyroid tissue, thyroid adenoma and papillary thyroid cancer tissue positive expression rate of 35.00% (14/40), 55.56% (20/36) and 88.33% (53/60) . Bcl-2 mRNA positive expression rate was significantly higher than that in papillary thyroid carcinoma thyroid adenoma and normal thyroid tissue, and the difference was statistically significant (P lt; 0.05). 6. Bcl-2 protein and mRNA expression in papillary thyroid carcinoma tissue and the patient's age, regardless of gender (P gt; 0.05), with lymph node metastasis related (P lt; 0.05). 7 papillary thyroid carcinoma tissue regardless of WWOX protein expression rate of Bcl-2 protein positive expression rate, or of WWOX mRNA positive expression rate of Bcl-2 mRNA positive expression rates by the spearman level analysis showed a significant negative correlation resistance (r = -0.298, P lt; 0.05). Conclusion 1. WWOX low expression in papillary thyroid cancer occurred related to the development. 2. Bcl-2 expression in papillary thyroid cancer occurrence and development. 3 of WWOX protein mRNA low expression of Bcl-2 protein, mRNA expression of thyroid papillary carcinoma lymph node metastasis. 4. WWOX during the development of papillary thyroid cancer with Bcl-2 may play an antagonistic effect.

The Expression and Significance of WWOX, Bcl-2 in Human Thyroid Papillary Carcinoma

Abstract

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