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The Study of the Difference of Period1 and Period2 Between Gloma Cell Line and Normal Cell Line Cultured in Vitro

Author: CaoShuanZhu
Tutor: XiaHeChun
School: Ningxia Medical University
Course: Surgery
Keywords: Circadian Rhythms Glioma Per1 gene Per2 gene
CLC: R739.4
Type: Master's thesis
Year: 2010
Downloads: 47
Quote: 1
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Abstract


The physiological activities of the plants and animals as well as human nature, have a day and night of the different living habits. This approximate 24-hour circadian rhythm is generated by the internal biological clock. Rhythmic oscillation of the circadian clock is composed of positive and negative feedback loop consists of a series of biological genes. Per1, Per2 as a core circadian clock gene which is critical in the generation and maintenance of circadian rhythm. Per1, Per2 gene mutation or deletion, expression abnormalities can cause biological circadian rhythm disorders or disappeared, followed the lead of the occurrence of the disease, or to promote the development of the disease, prompting premature aging, tumorigenesis. By transgenic technology to increase of Per1 the Per2 gene overexpression in tumor cells can inhibit tumor cell growth and promote tumor cell apoptosis. Malignant gliomas are the most common intracranial tumors of the central nervous system, difficult to total resection surgery, radiotherapy and chemotherapy side effects, you can not play a role. Research background and purpose of the study found that the occurrence of the clock genes of Per1, Per2 mutation or deletion in breast cancer, lung cancer and lymphoma and other malignancies, the development of a close relationship. Not yet concerned glioma between circadian clock gene-related research. The objective of this study is to investigate glioma circadian clock gene of Per1 relationship between Per2. Test Method for the cultivation of the cell line, PMA-induced cells produce circadian gene expression. Cells taken at different time points using immunohistochemical method and RT-PCR assay in vitro glioma cells and normal cells of Per1, Per2 differences in gene expression. Image pro-Plus results of immunocytochemistry results processing, analysis of variance showed that PMA-stimulated NIH3T3 cells induced Per1, Per2 gene expression showed a circadian rhythm, and PMA-stimulated C6 cells induced Per1, Per2 gene expression does not show for the circadian rhythm, NIH3T3 cells of Per1, Per2 genes Per1, Per2 gene expression in C6 cells there is a significant difference. Per1, Per2 genes in NIH3T3 cells and C6 cells using semi-quantitative RT-PCR to detect mRNA experimental results processing reached the same result. Conclusion C6 cells and NIH3T3 cells Per1 and Per2 gene expression. Per1 and Per2 gene was recently rhythmic expression in NIH3T3 cells, Per1 and Per2 gene expression in C6 cells do not exhibit a lower level for the circadian rhythm and expression.

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