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Cellulose Chiral Stationary Phase Preparation and synergies

Author: MengLei
Tutor: YuanLiMing
School: Yunnan Normal University
Course: Physical and chemical
Keywords: High Performance Liquid Chromatography C18 Cellulose derivatives beta - cyclodextrin Synergies of the two-phase
CLC: O657.7
Type: Master's thesis
Year: 2002
Downloads: 178
Quote: 4
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Abstract


In this paper, the following four aspects: 1. Briefly the significance of the chiral separation and liquid chromatography on chiral separation superiority. High performance liquid chromatography with chiral stationary phase of the development process, in particular cellulose derivatives in liquid chromatography, and to explore the mechanism of cellulose derivatives for chiral separation. High performance liquid chromatography high temperature does not occur is separated solute conformational changes or destruction of biological activity, the capacity of the column, has huge potential to develop into a laboratory and industrial scale separation technology mapping system equipment. Conventional chiral liquid chromatography separation is the case of the type of branch-type liquid chromatography stationary phase, mobile phase two lines, stars. We as a link to the target isolate, handle mobile phase chiral stationary phase combined investigation of chiral separation mode. 2. Preparation of non-chiral stationary phase - inverting key conjunct C 18 stationary phase packing and installed to fill the column. By commodity C 18 column, commodity C 18 filler loading column contrast to synthesize and filling in column performance, column efficiency and stability. And, erythromycin and methylerythromycin split (alpha = 2.1), showing good separation effect, indicating that the column can be used for the general inverting separation work. The study also examines the chiral separation of a model: the non-chiral stationary phase column was added chiral mobile phase additives - beta-cyclodextrin derivatives as chiral additives in C 18 column split on the part of the amino acid. 3. Continue to explore the optimization of synthetic cellulose derivatives on the basis of the the cellulose tribenzoate (a) was prepared and cellulose to three phenyl carbamate (b). Esterification of hydroxyl groups on the two chiral reagents Yunnan Normal University Graduate Thesis Cellulose Chiral Stationary Phase Preparation and synergies'> ?, glucose unit completely confirmed by IR and elemental analysis. Built from a different column compared various methods Built column separation difference, find a preferred method of coating and the coating amount. And column separation performance was tested, showing the chiral separation ability in the amino acid on the separation of the enantiomers. Chiral cellulose column have investigated on the basis of chiral separation ability of different concentrations of p-cyclodextrin, 2,6-dimethyl-Bu cyclodextrin and 2,3,6-trimethyl- cyclodextrin as chiral mobile phase additives in cellulose column as chiral compounds, experiments show that the chiral additive to be added the obvious split role in promoting the joint action of the chiral stationary phase and chiral mobile phase greater than the chiral additives and chiral stationary phase two chiral separation factors stand-alone situation to start separation of the simple sum, to which we put forward in HPLC chiral two-phase synergies, this effect is different from mixed synergies of the stationary phase in the GC separation, it relates to the synergies between the two phases - the stationary phase and mobile phase additives. And initially explore the mechanism of the synergistic effect of the two-phase supramolecular chemistry principle. The synergies of the two-phase liquid chromatography has not been reported. 4. Based on gas chromatography special selective mixed stationary phase having different substituents having a synergistic effect of two factors to consider synergies as well as on the benzene ring of the cellulose derivative, a cellulose derivative mixture was coated with a chiral column synergies Experimental , partial amino acid enantiomers were the result of the mixed stationary phase in this case the isolates showed negative synergies.

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Chromatographic analysis
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