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Objective: To observe the schedule changes into bone cells at different growth stages caveolin-1 expression , and to study the impact of caveolin-1 on the proliferation and differentiation of MC3T3-E1 osteoblast function . Methods : MC3T3-E1 cells cultured in containing β-glycerophosphate , ascorbic acid medium 18d, caveolin- western blotting method observed in cell cultures at different times ( 3, 6, 9, 12, 15, 18 d ) 1 protein expression changes . Down caveolin-1 expression by western blotting screening and confirmatory RNA interference plasmid pGenesil-1.1-cav-1 plasmid , to observe the impact of the proliferation and differentiation of osteoblast MC3T3-E1 : 3 days after transfection determination of cell viability (MTT) , respectively, after transfection 3,6,9,12,15 d ALP activity in cell lysate was measured (PNPP method) , the culture supernatant of osteocalcin concentration ( ELISA ) . : Caveolin-1 protein with the differentiation of MC3T3-E1 cells , expression levels gradually decreased each treatment group had no significant impact ( P gt proliferative phase MC3T3-E1 cell viability (P lt; 0. 01) ; 0 .05 ) . Transfection RNA interference plasmid pGenesil - 1.1 - cav -1 group , ALP activity and osteocalcin concentration with the control group compared to the peak significantly increased , and the peak forward . Conclusion : of caveolin- 1 on osteoblast MC3T3-E1 differentiation having a negative regulatory role , does not affect proliferation .
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