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The Role of 5-HT Involved in the Mechanism of Preventing Chemotherapy-induced Nausea and Vomiting of Forsythia Suspensain in Rat Pica Model
Author: WangJing
Tutor: NieKe
School: Shandong University of Traditional Chinese Medicine
Course: Chinese and Western medicine combined with the basis
Keywords: Forsythia Rats Cisplatin Chemotherapeutic pica 5-HT and 5-HT3A receptor
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 65
Quote: 0
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Abstract
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Objective: The use of chemotherapy rat pica model to explore the role of 5-HT mechanisms the forsythia to chemotherapy nausea and vomiting . Method : different clinical commonly used chemotherapy drugs cisplatin rat pica model of rats was measured daily feeding kaolin amount as nausea and vomiting indicators . 72 hours after injection of cisplatin using HPLC- fluorescence detection method for determination of serum and brain tissue 5 - HT ,5 -HIAA content , using immunohistochemical method of brain and intestinal tissue 5-HT ,5-HT3A receptor the immunoreactive cell research . Results: We have successfully established a rat caused by cisplatin different food addiction model , experimental results show : (1) small perforatum extract in large doses and phillyrin could significantly inhibit the acute phase rat pica ( injection of cisplatin 0-24h), forsythia extract large doses can inhibit delayed rat pica ( 24- 72h after injection of cisplatin ) . (2) Compared with model group , perforatum extract in large doses and phillyrin have lower serum and brain tissue 5 - HT and 5-HIAA content trend , but no significant difference . ( 3 ) forsythia extract small , medium, large dose and phillyrin of a significant reduction in the expression of 5-HT3A receptors in rat brain tissue , and the results are superior to ondansetron . Perforatum extract large doses can significantly reduce the 5-HT content of rat intestinal tissue perforatum extract in large doses significantly lowered 5-HT3A receptor expression in rat intestinal tissue . Conclusion: forsythia acute and delayed chemotherapy nausea and vomiting have a significant preventive effect and its mechanism of action related to the lower 5-HT content in brain tissue and small intestine and down 5-HT3A receptor expression . The phillyrin is one of the active ingredients the forsythia involved antiemetic , prevent acute chemotherapy nausea and vomiting , and its mechanism may be related with lower 5-HT content down 5-HT3A receptor .
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