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Hubei folk medicine bone Dan the sheath handle wood Cornaceae tooth sheath handle wood ( Toricellia an - gulata Oliv var intermedia (Harms ) Hu ) root , root bark , bark , leaves and flower genus treatable trauma fractures , bruises , rheumatism, joint pain , dry blood tuberculosis injury , postpartum low back pain , chronic enteritis , diarrhea , tonsillitis . The drug is widely distributed in Hubei Enshi folk and more with its treatment of rheumatism and joint pain , efficacy , and has broad prospects for development . This experiment, the large bone Dan 95% ethanol extract of petroleum ether and n-butanol portion was chromatographed to give 13 compounds . By physicochemical properties , a variety of spectral analysis technique , or directly with the standard control , identification wherein the Compound 12 are as follows: bis ( 4,7,8 - trihydroxy- 3 ,5 - dimethoxybenzaldehyde propyl ) -9 - ether ( 1 , new compounds ) , stigmasterol alcohol ( 2 ) , 3 , 4,5 - four hydroxyl groups - phenylacetic acid ( 3) , stigmasterol -5,11 - diene- 3β- ol ( 4) , 22 , 23 - dihydro stigmastan alcohol ( 5 ) , β - carrot glycoside ( 6 ) ,26,27 - dinorcholesta -8 ,14 - dien- 3β- ol (7) , hexadecanoic acid (8) , octadecanoic acid (9 ) , α-D - pyran - glucose ( ) -β-D - glucopyranose (11), 4 - hydroxy - 3 ,5 - dimethoxybenzaldehyde (12) . Compound 1 is a new compound ; compound 3 for the first time from a natural plant extract isolated ; compounds 1,2,3,7,12 are isolated from this plant for the first time . Six large bone Dan separated monomer compound 1,2,5,6,7,13 reaction experiment that occurs associated with RA immune activity test -Con A - induced proliferation of rat spleen cells . The results show that , by bone Dan separation resulting six compounds were compared with the control group, the Con A - induced proliferation of rat spleen cells have different degrees of inhibition , the difference was statistically significant ( P lt; 0.05 ) .
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