|
The purpose of looking for a new , convenient , and the effects of anti- immune rejection release agent , by extraocular topical treatment and prevention of corneal allograft rejection ; further clear collagen pellicles carrier FK-506 inhibits corneal allograft rejection reaction mechanism. Methods 36 New Zealand white rabbits ( 72 eyes) to establish a high-risk corneal transplantation animal model , then the purposes of penetrating keratoplasty to create corneal transplant rejection model ; all white rabbits were divided into 3 groups , slow release collagen pellicles upload FK506 experimental group , drops of FK506 eye drops ( control group 1 ) and slow release collagen pellicles upload ring cyclosporin a ( control group 2 ) , ( ??1) was measured by enzyme-linked immunosorbent assay (ELISA) method experimental group and control group 1 postoperative the 2,4,8,24,72 h cornea , conjunctiva, aqueous humor drug concentration and duration ; ( 2 ) the observed experimental group and a control group of corneal graft rejection and epithelial cells healing and pathological changes ; ( 3 ) . grafts in different periods of corneal transplant rejection incidence , electron microscopy the ultrastructure of corneal grafts , with an average follow - up of 3 months . Results The experimental group after the cornea, the conjunctiva, the aqueous humor concentration of the drug compared with the control group cornea, conjunctiva, high drug concentrations of the aqueous humor ; the cornea of the experimental group the highest drug concentrations , approximately 5 times the control group cornea ; experimental group, the drug concentration of the aqueous humor 72h still higher than the control group 8h of aqueous humor drug concentration is about 10 times larger ; corneal lymphocytes of the experimental group, the number of very small , while the control group of the cornea contains a large number of lymphocytes and eosinophils . Closely corneal endothelial nuclei Chengbian oblong , evenly distributed nuclear chromatin , abundant cytoplasmic organelles , cell microvilli visible connection between the transmission electron microscopy experimental group cells , the ultrastructure of the control group had been dissolved . Conclusion sustained release a collagen membrane uploaded FK506 can significantly improve and FK506 concentration for a long time to maintain the cornea , conjunctiva and aqueous humor effectively inhibit corneal transplantation immune rejection ; The drug metabolism comply with a pharmacokinetic , FK506 sustained-release collagen drug the film is a novel and effective method of administration .
|