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Aconitum pendulum the Ranunculaceae plant Aconitum pendulum Aconitum pendulum Busch flavum A. flavum Hand.-Mazz dry roots. Qiang name of the drug, Tiger bar half turn, alias snow artemisia, three-turn half. Sexual bitter, slightly acrid, hot, highly toxic. Stasis, rheumatism, pain, swelling and stasis effect, for the treatment of bruises, rheumatic pain, low back pain, malignant sore carbuncle, snakes and other injuries. Aconitum pendulum Qiang orthopedic commonly used drugs, efficacy, and long history of use. But the Qiang Aconitum pendulum herbs fried sand processing methods is not perfect, no quantitative standard processing technology, research on medicinal quality standards have not been reported. Objective: Ethnobotany method Aconitum pendulum medicinal conducted a survey on the basis of respect for national drug habit, Qiang medicine Aconitum pendulum chemistry, efficacy, toxicity study, specification Aconitum pendulum processing technology and the establishment of Aconitum pendulum medicine quality standards. Method: 1. Ethnobotany research methods to study the source of Aconitum pendulum base, distribution of national drug experience and processing methods; (2) the establishment of different processed products of RP-HPLC fingerprint comparison under different processing conditions, iron bars hammer raw products and processed products, and changes in chemical composition between the different processed products; 3. study Aconitum pendulum Health products and different processed products of acute toxicity, determination of LD50 of raw products and processed products of the maximum tolerated dose, evaluation Aconitum pendulum raw products and processed products toxicity; 4. xylene-induced mouse ear edema model, agar induced granuloma model to study iron bar hammer different processed products anti-inflammatory effects, using the mouse acetic acid writhing The law visits Aconitum pendulum different processed products analgesic effect. Comprehensive chemical fingerprint and Determination; well as anti-inflammatory and analgesic experimental acute toxicity test results, combined with the actual Pieces plant larger pilot specification Qiang Aconitum pendulum concocted experience through standardized processing technology to produce different origin, different harvest time of 17 batches of Aconitum pendulum sand fried processed products. By silica gel column chromatography and preparative TLC and spectral identification method of iron bars, hammers processed products of plant chemical research. Results: 1. The Qiang drug Aconitum pendulum is mainly distributed in Sichuan Aba Tibetan and Qiang Autonomous Prefecture in Mao County, Blackwater, Songpan, the original red mountain. With stasis, rheumatism, pain, efficacy, for the treatment of bruises, rheumatism, back pain, the malignant sore carbuncle, snakes and other injuries. Highly toxic, orally be concocted with raw products of iron bars, hammers, Qiang processing methods, including the method of sand fried, boiled method and steaming method. RP-HPLC fingerprint of different processed products, processed products do not differ in chemical composition, raw products and processed products, the more obvious differences in chemical composition. 3 of Aconitum pendulum Students goods toxicity, LD50 0.9605g/kg, equivalent to 14 times the clinical dose of adult day; maximum dose of processed products 17.4920 g / kg, equivalent to the clinical dose 262 times; processed products of the maximum dose of 16.8360 g / kg, equivalent to 252 times the clinical dose; the processed products maximum dose of 16.8400 g / kg, equivalent to 252 times the clinical dose. The results showed that the sand fried processed products of its toxicity were significantly lower. 4 different Aconitum pendulum processed products pharmacodynamic study Aconitum pendulum processed products have a certain anti-inflammatory and analgesic activity, the activity of three kinds of processed products size was not significantly different. 5. The Qiang drug Aconitum pendulum concocted the optimum: Take iron bars, hammers, cut to a size of about 1-2cm uniform segment, the amount of clean sand (particle size the Pharmacopoeia sieve and sieve, the amount of sand can be completely submerged herbs degrees) in medicine roaster drum heated to 200 ° C, the speed to medium speed (about 10 rev / min), to be reached temperature, put into good cut herbs, frying 5 minutes or so, to the skin brown, inner slightly yellow until 6. preliminary draft quality standards established an iron bar hammer raw products and processed products, including the identification, inspection, measurement of the content. 7 hammer processed products from iron bars isolated and purified five compounds: beta-Valley sterols (beta-sitosterol), unknown D unknowns A the benzoyl aconine (Benzoylaconine) 14 - phenylacetyl Ukraine head rather Conclusion: 1. the Qiang drug Aconitum pendulum processing methods for sand fried Law, sand fried processed products of the chemical, pharmacological and toxicity test results show that, concocted Aconitum pendulum herbs and good safety and good resistance to inflammatory analgesic activity, to achieve the purpose of highly toxic medicinal attenuated deposit efficiency. Developed Aconitum pendulum raw products and processed products of draft quality standards, in particular the determination of processed products, to ensure the safety of folk medicine clinical medicine quality, effective and controllable.
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