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Objective To observe Dicliptera polysaccharide in dimethyl nitrosamine (dimethylnitrosamine, DMN)-induced liver fibrosis in antagonism, as well as TGF-β 1 expression, further research and development Chinese medicine and its effective monomer for the clinical treatment of liver fibrosis provides a new way. Methods 80 Wistar rats were randomly divided into normal group, model group, Dicliptera polysaccharide high dose group and low dose group and the colchicine group of five groups of 16, male and female. 1st day of the experiment, in addition to the normal control group, the remaining rats in each group are 0.5% DMN solution 1.6ml/kg dose according to body weight by intraperitoneal injection per week for 3 consecutive days, 1 day, 1 week with 2/3 dose, after using full volume. Dicliptera polysaccharide high dose (0.3g/kg/d), low dose (0.1g/kg/d) and colchicine group (0.1mg/kg/d) were administered orally once a day, the normal physiological group I Brine, a total of four weeks. 4 weekend Abstract rat eyeball blood, weighing the liver, spleen and receive liver tissue wet weight; automatic biochemical analyzer serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and albumin (ALB) indicators; enzyme-linked immunosorbent assay (ELISA) of serum hyaluronic acid (HA), laminin (LN), Ⅲ procollagen (PC Ⅲ), Ⅳ collagen (Ⅳ-C); hematoxylin - eosin (HE staining) and Masson staining HF formation; Immunohistochemical detection of transforming growth factor β 1 (transforming growth factorβ 1 , TGF-β 1 ); Results ① liver: Dicliptera polysaccharide of high and low dose group ALT, AST, TBIL, ALB were significantly lower than the model group, the differences were statistically significant (P lt; 0.01). ② liver fibrosis four: Dicliptera polysaccharide high dose group, serum liver fibrosis four indicators (HA103.5 ± 8.67 ng / mL, LN87.3 ± 12.51ng/mL, PC Ⅲ 30.8 ± 3.68 ng / mL, and IV -C49.7 ± 4.56ng/mL) slightly higher than the normal group (P lt; 0.05); But with the model group (respectively HA182.4 ± 16.62ng/mL, LN142.6 ± 18.82ng/mL, PC Ⅲ 56. 3 ± 4.91ng/mL, IV-C88.9 ± 8.74ng/mL) compared Dicliptera polysaccharide of high and low serum liver fibrosis four dose groups were significantly lower (P lt; 0.05, P lt; 0.05, P lt ; 0.05, P lt; 0.05). ③ pathology HE and Masson staining: Dicliptera polysaccharide of high and low dose groups necrosis of liver inflammation and fibrosis severity was significantly reduced compared with the model group, the differences were statistically significant (P lt; 0.01). ④ liver tissue immunohistochemical staining: model group, TGF-β 1 mainly expressed in the portal area and fibrous septa, and the elongated oval or spindle, immunohistochemical staining area was significantly higher than the normal control group . Dicliptera polysaccharide of high and low dose groups TGF-β 1 expression was significantly decreased compared with the model group and normal control group, no significant difference. Conclusion 1 Dicliptera polysaccharide with anti DMN induced liver fibrosis, and its mechanism and protect liver enzymes and reduced liver tissue TGF-β 1 expression. 2, Dicliptera polysaccharide possibly through reduced TGF-β 1 expression, thereby inhibiting the activation of HSC, eventually leading to decreased synthesis of ECM and HA secretion decreased its anti-fibrosis.
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