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Infection Simulation、Pathogenesis Mechanism of Pseudoperonospora Cubensis and Induced Resistance by High Temperature in Cucumber

Author: ShiYanXia
Tutor: LiuXueMin
School: Northeast Agricultural University
Course: Plant Pathology
Keywords: Cucumber downy mildew Pseudoperonospora cubensis Infection analog Pathogenic mechanisms High temperature induced resistance
CLC: S436.421
Type: Master's thesis
Year: 2002
Downloads: 491
Quote: 10
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Cubensis is an infectious disease caused by the Cuban false downy mildew (Pseudoperonospora cubensis Rostow). More than 70 countries around the world, the great threat of cucumber production. After the 1980s, along with the expansion of the scale of the facilities for vegetable production, the the shed room temperature and humidity conditions conducive to the occurrence of disease prevalence, cucumber downy mildew of cucumber production obstacles. Effective against cucumber downy mildew and reduce the environmental pollution caused by chemical control for the purpose of ecological control of cucumber downy mildew, from the following aspects for were discussed. Cucumber downy mildew fungus infection simulation study, clear their infection temperature and humidity conditions, downy mildew infection detached leaves optimum temperature is 25 to 35 ° C for 2h and humid enough to cause infection. -20 ℃ cryopreservation 10 months cucumber downy mildew strains still have virulence. Temperature affect the virulence of the infection model, to demonstrate compliance Logistic model. Produce sporangia in vitro and in vivo disease leaves can be several days in a row, analysis of significant disease days after cumulative sporangia of parabolic type found between the significant disease the number of days between the lesion area in line with changes in Gompertz mode. First proposed cubensis pathogenic mechanisms produce pathogenic toxins and pathogenic enzymes extracted to a higher activity of pathogenic toxins SM-2. The hot stuffy shed Combat cucumber downy mildew proposed twenty years, have not yet carried out a detailed study of its associated environmental factors. Greenhouse controllable high temperature conditions, humidity pathogens lethal impact at home and abroad also no system reports. This article take the combination of temperature and humidity to study high-temperature processing cubensis infection clear under the conditions of 80% relative humidity, 45 ° C for 2h can effectively prevent the occurrence of cucumber downy mildew, which for ecological control method provides a new theoretical basis for field application. While the high-temperature-induced host - cucumber can also produce resistance, this conclusion is the innovation of this study. Study the mechanism of high-temperature induced resistance, and found the host after the high-temperature-induced PAL, POD, SOD, chitinase and of β-1, 3 - glucanase activity increased compared with the control. Cell wall surface has a large number of lignin deposition, analysis of several factors that, in the high temperature during the induction of host defense enzymes and lignin collaborative play Resistance chitinase and β-1, 3 - Portugal xylanase degradation of downy mildew sporangia cell wall, the to destroy germs organization to play Resistance. Theories and methods of this study provide further ecological control of cucumber downy mildew.

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Horticultural Crops Pest and Disease Control > Vegetable pests > Melons, pests and diseases > Cucumber pests and diseases
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