Dissertation > Excellent graduate degree dissertation topics show

Study on Natural Infection with Ovine Lentivirus (Southern Xinjiang Strain) in Xinjiang Karakul Sheep

Author: ZhangHui
Tutor: AHeMaiTi·MaiMaiTi
School: Xinjiang Agricultural University
Course: Basic Veterinary Science
Keywords: Xinjiang Kalakuer sheep Ovine lentivirus(southern Xiniang srtain) agar gel immunodifusion (AGID) mild lymphocytic mastitis proviral DNA
CLC: S858.26
Type: Master's thesis
Year: 2005
Downloads: 48
Quote: 0
Read: Download Dissertation

Abstract


1631 sera of Xinjiang karakuer sheep collected from the Southern Xinjiang were tested for antibody to ovine lentivirus(OvLV)(southern Xiniang srtain) by agar gel immunodiffusion(AGID).the results showed that the average positive rate was 1.3% and Xinjiang Kalakuer sheep is a hyposensitivity breed to OvLV in serology. 14 adult Karakuer sheep(female=12,male=2) from sheep farms in southern Xinjiang that were naturally infected with OvLV(southern Xiniang srtain) were studied in this paper. The sera of the 14 selected seropositive sheep were consecutively tested for 18 months(once a month), the results showed that the virus antigen was drift. None of evident clinical signs and OPP-specific gross lesions were seen in all seropositive sheep. Histologically, the lymphocytic interstitial pneumonitis (LIP), vasculitis and arthritis/synovitis were not found in all seropositive sheep, the only typical OPP lesion was of mild lymphocytic mastitis. The result showed that the OvLV strain of Xinjiang Karakul sheep is a hypovirulent strain and Xinjiang Karakul sheep is a hyposensitivity breed to OvLV in pathology. 7 sera from Xinjiang Kalakuer sheep were tested by AGIDT. Ovine lentivirus gag gene that from peripheral blood cells of 5 tested sheep was used as template and amplified by PCR. Then the amplified fragments were cloned into pBS-T vector. The recombination product was transformed into E.coli DH5α.The positive recombinant was screened on Amp/IPTG/X-Gel plates and identified by restriction endonuclease XhoI, clony PCR and then sequencing. The result shows that the PCR are more sensitive than AGIDT and there is some homologue between the gag gene sequence of OvLV (southern Xiniang srtain) and Visna virus.

Related Dissertations

  1. The SHIV-XJ02170 infected Chinese rhesus monkey experiments,R-332
  2. Detecting of Equine Infectious Anemia Virus Proviral DNA in Peripheral Blood Monocytes of Animals Inoculated with Live Attenuated Vaccines and the Infectious Molecular Clone Derived Strains,S852
  3. Molecular Mechanism of Brucella Menlitensis Infecting Embryonic Trophoblast Cells,S858.26
  4. Study on Developping of Peste Des Petits Ruminants’s ELISA and PCR Test,S858.26
  5. Studies on the Clinical and Molecular Pathology of Sheep with Naturally and Experimentally Infected Pulmonary Adenomatosis,S858.26
  6. Investigation and Control on the Major Epidemic Diseases of Goat and Sheep Farms in Hebei,S858.26
  7. Biological Characterization of Theileria spp. Infective for Sheep and Goats in China,S858.26
  8. The Finding of Lamb Encephalitis Induced by Enterococcus and Studies on Its Pathogenic Characteristics and Diagnosis Method,S858.26
  9. Preliminary Study on Anthelmintic Resistance in Gastro-intestinal Nematodes of Small Ruminants,S858.26
  10. Establishment of Immunohistochemistry Assay for Scrapie Detection and Characteristics Research for Mab 2H3 Which Identify Ovine PrP from Bovine PrP,S858.26
  11. Study on the Molecular Mechanism of Pathogenesis for Brucella Ovis,S858.26
  12. The Epidemiology and Prophylaxis-Therapy of Mycoplasma Ovipneumoniae Infection in Goats and Sheep,S858.26
  13. The Monitoring of Placental and Cervical Ripening in Xiao Wei Han Sheep at Mid and Late Periods of Gestation,S858.26
  14. Preperation of Sheep Pasteurella Haemolytica Deactivation Vaccine and Its Immunity Efficacy Measurement,S858.26
  15. The Investigation and Chemical Prevention of Ovine Theileriosis in Ganan Tibetan Autonomous Region,S858.26
  16. Biological Characteristics of Mycoplasma Ovipneumoniae and Its Rapid Detection by Using Polymerase Chain Reaction,S858.26
  17. Artificial Infection Experiment and Establishment of the PCR Method for Detection of Theileria sp,S858.26
  18. The Preliminary Study on Natural Infection with Ovine Lentivirus in Xinjiang Karakul Sheep,S858.26
  19. The Study of the Correlation between Sheep Feed in Shed and Selenium Deficiency,S858.26
  20. The Investigation of Pathological Changes and Pathogenesis of the Pregnancy Toxemia of Sheep,S858.26

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Livestock > Sheep
© 2012 www.DissertationTopic.Net  Mobile