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Functional Identification of SDKB Gene in Rice Through RNA Interference

Author: LeNing
Tutor: FengJiaXun
School: Guangxi University
Course: Biochemistry and Molecular Biology
Keywords: Arabidopsis thaliana NPR1 gene SDKB gene RNAi rice disease resistance quantification PCR
CLC: S511
Type: Master's thesis
Year: 2005
Downloads: 191
Quote: 2
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Abstract


The Arabidopsis thaliana NPR1 is a positive regulator in systemic acquired resistance (SAR). To identify the function of SDKB, an Arabidopsis thaliana NPR1 homologous gene in rice, an RNAi rice expression vector pCAMBIA 1301-ihp for silencing SDKB was constructed. Rice cultivar Gui99 was transformed with pCAMBIA 1301-ihp by Agrobacterium tumefaciens-mediated transformation. Six hygromycin resistant regenerated seedlings were obtained. They were confirmed to be transgenic plants through PCR and Southern hybridization analysis. The result of RT-qPCR revealed that the SDKB expression level in the T1 progeny of three transgenic plants was 16.15%, 3.49% and 15.07% of that in wild-type plant respectively, showing a significant suppresssion of SDKB expression. The Tl plants of the three transgenic lines developed a lesion length which was 1.7, 1.9 and 2.3 folds to wild-type plant respectively after challenged with Xanthomonas oryzae pv.oryzae and had a disease index which was 1.4, 1.7 and 1.6 folds to wild-type plant respectively after inoculated with Magnaporthe grise, showing an increased susceptibility to both Xoo and M.grise. These results confirmed that SDKB played a positive role in rice disease resistance regulation and had a potential application value in disease resistance improvement for rice and other crops.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
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