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Effect of CdCl2 on Sperm Toxicity and Sex Ratio in Mouse and Bovine

Author: YuRong
Tutor: YangLiGuo
School: Nanjing Agricultural College
Course: Animal Genetic Breeding and Reproduction
Keywords: Cow Mice Sperm Embryos Reproductive toxicity Sex identification
CLC: S814.7
Type: Master's thesis
Year: 2005
Downloads: 163
Quote: 1
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Abstract


In this study, the same amount from the two angles of the in vivo and in vitro study cadmium on mice and cattle male reproductive function, in vitro fertilization, embryo development impact; bovine and mouse IVF embryos and Y-chromosome-specific primer PCR amplification, carried out The early embryo sexing of cadmium on embryonic sex differentiation in vitro fertilization. The results show that: the amount of per kg body weight of 0, 0.5, and 2mg (cadmium), mice were given subcutaneous injection of cadmium chloride daily, for five consecutive weeks. The anatomy study found mice body weight and testicular weight decreased, indicating that cadmium on mouse growth and reproductive have toxic effects, dose-dependent. Check semen quality and found a downward trend in semen quality in mice with cadmium injection dose and time increase, showed a decline in sperm concentration and sperm motility, sperm deformity rate (P lt; 0.05); When cadmium injection dose reached 2mg/kg epididymis weight, almost no live sperm, the show that has no apparent toxic effects of cadmium on the generation of mouse sperm quality. Above cadmium treated mice breeding and farrowing research found that the reduced ability of cadmium treated male mice breeding, the females breeding success rate decreased (P lt; 0.05); calving birth weight lower than the control group , decreased litter size. 2 during capacitation in bovine sperm in vitro, the cadmium content of 0,1,2,4 mg / L of sperm floating liquid treatment to study the impact of cadmium on bovine semen quality. The results showed that cadmium processing time and the dose will affect semen quality, and with the increasing doses of cadmium processing time and cadmium treatment, declining sperm motility, sperm deformity rate upward trend; compared with the control group, the high-dose group was significantly different (P lt; 0.05), showed a dose-dependent and time-dependent relationship. Cadmium content 0,1,2,4 mg / L of sperm floating liquid bovine and mouse sperm capacitation in vitro in vitro fertilization, to study the effects of cadmium on in vitro fertilization capacity and early embryonic development. The results show that: each test group, the fertilization rate was significantly lower than the control group compared with the control group, low-dose group differences are not significant with (P lt; 0.05), medium, and high dose group differences significant with (P lt; 0.05), showed that cadmium no apparent toxic effects on mammalian sperm in vitro fertilization; and the greater the dose of Cd toxicity enhanced role. The early development of embryos in research after fertilization, found cadmium apparent toxic effects of bovine and mouse early embryonic development, and inhibit the division and development of the early embryo, lower blastocyst formation rate. Sperm after treatment with cadmium in vitro fertilization, embryo cleavage rate lower than the control group (P lt; 0.05), 8 - cell rate, Sang the blastocyst rate also decreased significantly (P lt; 0.05), malformed embryos during development increase in a dose dependent manner. Inferred mainly due to cadmium sperm within a variety of enzymes and DNA damage and injury, leading to slow the development of embryos after fertilization and abnormal. 4 per kg body weight of 0, 0.5 and 2mg/kg · W (cadmium), daily subcutaneous injection of cadmium chloride in mice 1 for five consecutive weeks after breeding, research Cd pups gender differentiation impact. The results showed that cadmium treatment Aberdeen

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > General Animal Science > Livestock and poultry breeding > Farrowing and lactation
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