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Prepubertal testicular damage diseases, such as cryptorchidism, testicular torsion, varicocele line spermatic vessels ligation, incarcerated inguinal hernia, the more common clinical tumor compression spermatic vessels and surgical resection of testicular vascular injury. For cryptorchidism, in which about 30% for high cryptorchidism, often due to the spermatic vessels is too short to be Fowler-Stephen surgery (high ligation of spermatic vessels cut long loop vas deferens testicular fixation), but the rate of postoperative testicular atrophy up to about 30%. In the Adult-line variety of testicular surgery by semen analysis, determination of FSH and testosterone, testicular biopsy and other methods to determine the status of testicular function. In children, the current method can only be applied in the clinical chorionic gonadotropin (hCG) stimulation test, but the method can only be partly reflects testicular interstitial cell function, can not understand before puberty the most dynamic the seminiferous tubules part of functions. Therefore it is necessary to find a more objective assessment of prepubertal testicular function, to keep track of observation and evaluation of postoperative changes in testicular function. The inhibin (Inhibin) is a different dimer glycoprotein hormones, protein and its receptor superfamily members belonging to the transforming growth factor beta (TGF-beta), alpha and two beta subunits (βA and βB) one connected together via a disulfide bond, i.e. inhibin A (alpha-βA), and inhibin B (alpha-βB). In the male body, the main physiological form of inhibin inhibin B, male life could be measured serum inhibin B. Inhibin B are members of an important component of the hypothalamic - pituitary - gonadal axis. FSH stimulates the testes to produce inhibin B, and inhibin B negative feedback inhibition of pituitary secretion of FSH. In adults, the cellular localization of inhibin B, two in 2005, Zhejiang University School of Medicine Thesis sub units generated has not been determined, but it suppresses the hormone B and FSH negative feedback relationship has been clear, and serum inhibin Su B has been applied Analyzing the diagnosis of male infertility and testis function in children, its application is not yet conclusive. The purpose of this study is to establish the rat testis before puberty ischemic injury model by observing the serum inhibin B levels and their PB subunit mRNA expression level changes in the cells of the testis, and testis ischemic injury morphological changes and serum FSH, LH changes to evaluate serum inhibin B can reflect the change in functional status of the testis after the ischemic injury of the testis before puberty. Materials and side of the male 25-day-old Wista: 72 rats were randomly divided into control group and the spermatic vessels ligation group (referred to as the ischemic group). The animals were anesthetized, the control group into the abdominal cavity after surgery light Tizhao pill as soon as the abdomen was closed: ligation group to the right side of the high ligation of the spermatic vessels. In 1,3,5,7,9,20 postoperative days (group 6) anesthetized venous blood to the eye 2-3ml, after centrifugation serum inhibition of the hormone B (double antibody enzyme-linked immunosorbent assay ), FSH and LH levels (chemiluminescence); Caesarean section was cut bilateral testicular conventional histological examination tissue fixation HE staining and the Inhibin B 2011 B subunit mRNA by in situ hybridization observed in the testis within various intracellular expression. Received the various data SPSSIO.O statistical package for statistical analysis. Results HSLV death after three. Testis appear shrinking. Postoperative serum FSH, in the control group (10.56 ± 1.17 ligation group, 7,9,20 days after 1,2,6 9 ligated side, inhibin B levels: after 9 days, 9. 02 with disabilities 0.53mIU/ml, P (0.001), the ligation group FSH levels higher than LH there was no detectable change (0.48 ± 27 vs 0.45 Shi 0.29 mIU / ml, p gt; 0.05); ligation group inhibin B level significantly lower than the control
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