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Cell sensor is a hot topic in biosensors , has recently been widely used in drug screening , environmental monitoring, endotoxin detection . There are many such the FET ( fie1d effecttransistor ) microelectrode cell action potential measurement - based sensor technology live cells were cultured in the sensor surface , in cell action potential drugs or electrical stimulation , the signal through the gap between the cells and sensors coupled to the The sensor was detected . The type of sensor can not only detect a single cell in drug stimulation response to drug screening and in cultured cells of the network, is used to study cell network signal transduction pathways , transmission speed sensor array . FET and MEA due process as well as lead and other issues , a single measurement unit can not be made very small, and it is difficult to do individual cells fall on a single measurement unit , affecting the measurement . LAPS simple fabrication process , the measurement points can be achieved through the movement of the light spot . Therefore , we proposed based on a the LAPS single cell sensor , by measuring cells to evaluate the drug in drug stimulus response . The main content of the subject : 1. Analysis the LAPS equivalent model spatial and temporal resolution , voltage resolution , drawn to select the thickness of the epitaxial layer 5μm 10 Ω cm resistivity epitaxial wafers as the base , so that the sensor can achieve single-cell measurement purposes . 2 with poly- ornithine and laminin coated phosphate buffer treatment LAPS surface , enhance cell adhesion , greatly improving the sensitivity of the sensor . 3 . Built experiment platform , The hardware mainly by injection system , observation platforms , detection system . Software Labview, write the pump driver and the acquisition card program , autosampler and real-time detection . 4 is 2mv frequency with a peak-to-peak 1kHZ sinusoidal signal analog cell action potentials and as a sensor input signal , and the results show that the sensor is capable of detecting the signal . 5. 1 mg / l , the concentration of 10 mg / l two acetylcholine (acetylcholine, Ach) drugs as the stimulation of the cerebral cortex nerve cells detect the action potential of nerve cells in different concentrations under . Preliminary drug test results indicate that the sensor is able to detect different concentrations of acetylcholine .
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