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Objective: To observe the therapeutic effect of artesunate on rat adjuvant arthritis ( Ajuvant Arthritis AA ) its AA serum TNF -alpha , IL - 1 ? Content and apoptosis of synovial cells , Bcl-2 / Bax gene expression . : Select weight of 150 to 180g male Wistar rats , measuring the volume of bilateral paw , its right paw routine disinfection intradermal injection of complete Freund's adjuvant ( 0.1 ml / only ) to establish the AA model , made mode after 19 days , there will be secondary reactions rats were randomly divided into model group , artesunate treatment group ( 80mg/kg · d ) , Tripterygium wilfordii group ( 8 mg / kg · d ) ( n = 8 ) , other randomly selected eight normal Wistar rats was the control group , the rats paw volume with the volume measuring instrument on the date of measurement . Treatment groups for gavage for 14 days. Measuring the rats paw volume in the first 15 days after administration , the orbital blood , centrifuged, and serum , radioimmunoassay analysis measurement of serum TNF - alpha and IL - 1 ? Content . The conventional cultured rat synovial cell line , when transmitted to the 4th generation , with a 96-well plate artesunate on apoptosis of synovial cells the best drug concentration and the optimal duration of action is determined by the MTT assay . The logarithmic growth phase cell suspension was added to 24-well plates , 37 ° C cultivate 24 h , the proportion of 200μg/ml artesunate , and set equal parts positive control . Incubated at 37 ℃ for 24h , the specimens were fixed with 4% paraformaldehyde fixed , TUNEL assay apoptosis of synovial cells and immunohistochemistry to detect Bcl-2 and Bax . Results: 15 days after administration measuring paw volume : (1) modeling side of the mean degree of swelling (%) : model group 177.00 ± 37.5 , Artemisia annua
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