Dissertation > Excellent graduate degree dissertation topics show

The Role of Oxidative Macrophages (OMp) and Reductive Macrophages (RMp) in DSS Induced Experimental Colitis of Mice

Author: ZhangJing
Tutor: HanYing
School: PLA Postgraduate Medical School
Course: Department of Gastroenterology,
Keywords: Macrophages Experimental Oxidized Also prototype Acute enteritis Mice Mucosal injury Inflammatory cells Enteritis disease Oxidative Stress
CLC: R574
Type: Master's thesis
Year: 2005
Downloads: 79
Quote: 0
Read: Download Dissertation

Abstract


Inflammatory bowel disease (IBD) is a chronic inflammatory disorder but its etiology has not been clearly understood. Immunodysfunction has been considered as the prominent factor in its pathogenesis. Macrophages might play a key role in immunologic mechanism of IBD as abundant inflammatory cells infiltrating from patients’ inlamed gut, including macrophages and neutrophils. Gastrointestinal mucosa is endowed with several antioxidant defense systems, including the reduced glutathione (GSH) focusing on neutralizing the damaging effects of ROS. GSH plays a major role in gastrointestinal mucosal cytoprotection against oxidative stress in a variety of models. Indeed, previous studies examing the status of antioxidants in mucosa from IBD patients and experimental animal models of colitis have reported a decrease in the level of antioxidants, including that of GSH.According to Murata Y at el , macrophages can be divided into two classes basing on intracellular GSH level, namely the reductive macrophages (RMp) with high expression of intracellular concent of GSH and the oxidative macrophages (OMp) with less content of GSH. Several other studies reported that Crohn’ disease (CD) is classified as RMp/Th1 type response but OMp/Th2 type is associated with inflammatory cells infiltration. However, it isn’t clearly known about the transformation of GSH of local colon and immune cells in Dextran Sodium Sulphate (DSS) induced colitis and its relationships with mucosal injury and cytokine profile. In this study, we explore the expression of reduced glutathion (GSH) of colonic mucosa and GSH/GSSG within celiac macrophages in DSS-induced acute colitis and the relationship between GSH transformation and Th1/Th2 cytokine profile- IL-4 and IFN-γ as well as mucosal injury. Inaddition, to explore the role of macrophages in the pathogenesis and development of this type of colitis.1.Characteristics of Glutathion of inflamed colon in Dextran Sodium Sulphate induced acute colitis of mice and its relationships with mucosal injury and production of IFN-γand IL-4 .Methods BALB/C mice were fed with 5% DSS for 7days to induce acute experimental colitis, the colonic mucosa were checked histopathologically and GSH expression was evaluated with GSH1 Ab ; the expression of IL-4 and IFN-γ was also analyzed with ELISA. Results acute colitis was confirmed histopathologically in DSS group characterized by focal crypt lesions with granulocytes and macrophages in mucosa and submucosa. The level of GSH in DSS group was downregulated compared with that of control group. An increased IL-4 and a decreased IFN-γ were noticed. Conclusion Low expression of GSH is related with the increased IL-4 /decreased IFN-γ as well as mucosal injury in DSS-induced acute colitis of mice.2.The relationship of GSH/GSSG within celiac macrophages with mucosal injury and cytokine profile- IL-4 and IFN-γ in Dextran Sodium Sulphate-induced acute colitis of miceMethods BALB/C mice were fed with 5% DSS for 7days to induce experimental colitis, and macrophages in abdominal cavity were collected, the GSH, GSSG and GSH/GSSG in celiac macrophages were determined by Glutathione Assay Kits. The colon was isolated for both histopathological study and evaluation of IL-4 and IFN-γ as well as GSH expression. Results. The expression of GSH in celiac macrophages was downregulated but GSSG upregulated in DSS group, therefore the ratio of GSH/GSSG decreased significantly compared with that of control group. A notable increase of IL-4 in colon and a decrease of IFN-y in spleen were noticed in the DSS-induced colitis .Conclusion The type of celiac macrophages in DSS-induced colitis was

Related Dissertations

  1. The Realization of Coal Liquefaction in the Continum Model System and the Experimental Research of Coal Liquefaction,TQ529.1
  2. Research on HID Lamp Rectifying Effect,TM923.32
  3. Development of Experimental Robot Control System Based on ARM,TP242.6
  4. Research on the Teaching of Ditransitive Construction in Senior Middle School in the Perspective of Construction Grammar,G633.41
  5. Study of Sepia Ink-Astragalus Preparation for Relieving Damage Caused by Chemotherapy,R285.5
  6. Outward Bound to the introduction of the experimental study of junior high school students sunshine sports,G633.96
  7. Research on New Method for Measuring Dielectric Constant of Microwave Samples,O441.6
  8. The Analysis of Lolita Fashion Style Prototype and Its Application,TS941.2
  9. Broken walnut shell force testing and virtual prototype design of the main components of walnut sheller,S226.4
  10. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Immature Mouse,S865.13
  11. Study on Gene Expression of β-defensin1 in Mammary Gland of Mice,S852.4
  12. Experimental animal welfare legislation,D922.68
  13. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  14. Some Pharmacology and Toxicity of Three Prescriptions from Chinese Veterinary Pharmacopoeia,S859.5
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. Apolipoprotein E Gene Knock-out and High-fat Diet on IP3、 IP3R-1 ＆ Aβ Expression in Neurons of Mice’s Hippocampal CA1 ＆ Ca3,R329
  17. Effects of TACI-Ig on MRL/lpr Mice and Activation of JAK1-STAT1 Signaling Pathway in Lupus Nephritis,R363
  18. The Investigation on the Renal Injury and Effects on Oxidative Stress of Guinea Pigs Induced by Trichloroethylene,R363
  19. The Changes of Intimal Macrophages, Smooth Muscle Cells, Collagen, and Cpt-Ⅰ, GLUT-4 of Vascular Media in Human Lower Limb Artery with Atherosclerotic Stenosis,R543.5
  20. Effects and Some Mechanisms of TACI-Ig on Immune Function in MRL/lpr Mice,R593.241
  21. Maternal Exposure to Fenvalerate in Embryo Accelerated Age-related Changes of Behaviors in CD-1 Mice,R714.2

CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Bowel disease
© 2012 www.DissertationTopic.Net  Mobile