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Isolation and Identification of Greater Epithelial Ridge in Rat Cochlea

Author: SongWei
Tutor: DiSuoQiang
School: PLA Postgraduate Medical School
Course: Otorhinolaryngology
Keywords: Cochlea Greater epithelial ridge Separate Appraisal
CLC: R764
Type: Master's thesis
Year: 2005
Downloads: 23
Quote: 0
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Abstract


Generally considered noise, ototoxic drugs, aging and various diseases caused by hair cell loss, degeneration, injury is the sensorineural deafness main reason. But the hair cells are located in the inner ear sensory epithelia of terminally differentiated cells, previous studies have shown that mammalian cochlear hair cells after injury is not renewable. For a long time people in order to investigate the hair cell development, differentiation, regeneration mechanisms made unremitting efforts to stimulate the regeneration of hair cells to repair damaged hearing and balance disorders has been the main research directions. Regeneration may be repeated in the normal development process of hair cell differentiation. Therefore, understanding the differentiation of hair cells contribute to regeneration of hair cells. Mammalian vestibular hair cells also occurs in a similar manner with the lower vertebrates within the sensory epithelium derived precursor cells and supporting cells, but mammalian cochlear hair cells is unclear the exact origin, histological studies have shown that the inner and outer hair cells during embryonic development may respectively originated in large and small epithelial ridge. Recently, a large epithelial ridge (greater epithelial ridge, GER) mammalian hair cell regeneration become a new hot spot. Research shows that the greater epithelial ridge hair cells is probably one of the group precursor cells. Hair cell differentiation positive regulation of gene Math1 in cultured rat cochlea overexpressed, can induce the formation of a large number of freshmen greater epithelial ridge of hair cells. Some involved in hair cell differentiation and regeneration of important genes and growth factor receptors such as P27, Hes1, Math1 and FGFR1, etc. in different periods of greater epithelial ridge expression, these findings indicate that a large ridge in the hair cells of epithelial differentiation and regeneration process plays an important role, it is important to cell populations, there may be a precursor of cochlear hair cells one cell pool. Current understanding of much greater epithelial ridge, the subject aims to explore the use of enzymatic digestion and mechanical method of combining anatomical isolated the active greater epithelial ridge. By morphological comparison, immunohistochemistry, RT-PCR on the isolated large epithelial ridge identification, primary cultured to verify the separation of large epithelial ridge activity. A rat cochlear greater epithelial ridge separating selected postnatal day 0 SD rat cochlea, remove the basement, put it with thermolysin and a little DNA enzyme D-Hank's solution, 37 ℃ incubator and incubated 25 minutes for enzyme digestion, and then under a microscope at high mechanical separation. After the action of enzymes, the cochlear epithelial tissue and connective

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CLC: > Medicine, health > Otorhinolaryngology > Otology,ear disease
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