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Sichuan Angelica Quality Evaluation of standardized planting

Author: ZhongShiHong
Tutor: MaYuYing
School: Chengdu University of Traditional Chinese Medicine
Course: Pharmacognosy
Keywords: Sichuan Angelica Fingerprints Coumarin HPLC UV spectrophotometry Morphological and histological
CLC: S567.2
Type: Master's thesis
Year: 2005
Downloads: 202
Quote: 2
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Abstract


In line with the production quality management standardization Sichuan Angelica research paper based on previous work in the research group conducted a quality improvement and in-depth evaluation studies for the establishment of Sichuan Angelica standardized quality evaluation criteria provide a scientific basis. This paper first examines the river Angelica size and quality of the relationship between medicine, the main component measurement results show that the root of fine herbs (Third grade) good quality, it is recommended in the development of medicinal products grading standards combined with the intrinsic quality evaluation; first section for fresh Sichuan Angelica were observed and found that the proportion of bark and section diameter is inversely proportional to the angle from the morphological and histological explain third-class products with higher levels of the active ingredient reasons; first time Nakagawa Angelica stems, leaves for a more detailed organizational studies to identify the stems and leaves of main distinguishing characteristics. Key observation of the presence of secreted forms of organization and distribution, found scattered in the secretory ducts veins, petioles and stem cortex, phloem and pith of parenchyma. The use of \Heavy metal content of the sample measured all below 10ppm; arsenic salt content in all less than 2ppm. Angelica herbs for the River Determination of heavy metals and harmful elements and limited choice of available reference standards. HPLC samples were different Angelica Imperatorin, isoimperatorin and hydrated oxide decursin three kinds of coumarins determination, analysis and comparison of the measurement results for four consecutive years, combined with the actual production recommended determination Sichuan Angelica herbs imperatorin (C 16 H 16 O 4 ) and isoimperatorin (C 16 H 14 O 4 ) two kinds of coumarin, the total amount of not less than 0.12%. UV spectrophotometry was established medicinal Sichuan Angelica total coumarin approach. Select the 300 nm as the measurement wavelength. In Imperatorin as control samples for different Sichuan Angelica total coumarin content. The method is accurate, precise and easy to operate, fast and can be used for quality control of medicinal Sichuan Angelica. Analysis of measurement results, we recommend that the total content of coumarin Imperatorin, shall not be less than 0.6%. This is the first HPLC method for herbs for Ding Chuan Angelica coumarins fingerprint. Established the Sichuan Angelica coumarins fingerprint analysis. The sample is divided into Sichuan Angelica smoked two types of sulfur and sulfur is not smoked, were calibrated peaks. Use this article can be used to establish the fingerprint identification Angelica herbs are smoked sulfur. Angelica on standardized cultivation in Sichuan study different test samples were examined with a total peak area for quality analysis, the results consistent with the determination to improve the quality of Sichuan Angelica evaluation. Fingerprint analysis established stable, reliable, reproducible, and can fully reflect the overall sample coumarins case can be used as Sichuan Angelica quality evaluation methods of controlling them. The sauce on the River Angelica coumarins fingerprints were analyzed, the results showed that fingerprints sauce all the major peaks consistent with herbs, instructions coumarins mainly in the secretory tissue.

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