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Analysis on Flavone and Heredity of Agronomic Character of Tartarty Buckwheat Mutants

Author: FanLinHua
Tutor: LiGuoZhu
School: Shanxi Agricultural University
Course: Biophysics
Keywords: Bitter buckwheat Mutant Flavonoids Genetic variation Esterase isozymes HPLC
CLC: S517
Type: Master's thesis
Year: 2005
Downloads: 87
Quote: 0
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Abstract


The test three buckwheat varieties of flavonoids on 60 Co-γ-ray-induced mutations the body M 3 -and M 4 generation of genetic variation were studied, designed to improve the genetic mechanism of the flavonoid content of for buckwheat radiation mutagenesis and their descendants to provide a scientific basis; flavonoids were analyzed, in order to provide a scientific basis for the comprehensive development and utilization of buckwheat resources. The study found: (1) measurement results of genetic parameters of each mutant agronomic traits: plant height, stem branch number, grain weight has the high genetic force h 2 (%), respectively 84.85,76.36,90.33; grains per plant, grain weight per plant, main stem, number of branches genetic variation coefficient, respectively 20.13,16.06,19.33; grain weight per plant, number of branches of main stem highest genetic progress. (2) for each mutant GENETIC Correlation and path analysis of the results showed that: each trait grain weight per plant, number of branches of main stem, seed number per plant, grain weight and grain weight per plant was more large positive correlation, indicating that these traits have a higher genetic progress for the increase of grain weight per plant, and the main stem, number of branches and 1000 have a higher heritability (76.36% and 90.33%, respectively), can be used as plant grain weight indirect selection traits. (3) analysis of the flavonoid content and agronomic traits: plant height, number of nodes on main stem, number of branches of main stem and seed number per plant flavonoid content was positively correlated with plant height and flavonoid content was highly significant positive related, which means the vast majority of species of plant height higher the higher the flavonoid content. Weight and flavonoid content was a significant negative correlation means that the vast majority of varieties of grain weight higher flavonoid content, the lower, in accordance with this relationship in the breeding of high flavonoid mutants Destination Select plants is relatively high weight lower materials, the possibility of high flavonoid content varieties. (4) M 4 generation of mutant and control Isozyme Analysis of the results showed that: the mutant enzyme spectrum and control are some differences, in particular, changes in agronomic traits mutant, such as A 2 B 4 , the D 3 , the band width, the number and the color depth significantly changed, co-workers The enzyme is a direct product of the gene expression, mutation and genetic traits, agronomic traits of each of the mutants with the parental significant difference is caused by mutations. (5) the photometric determination of total flavonoid content of the mutant and parent, The results show that: M 3 -and the M 4 -generation grain total flavonoids than the control and M 3 and the M 4 little change in content of flavonoids in the generation of grain, buckwheat flavonoids content increased, the hereditary is relatively stable, indicating that the radiation leads to genetic variation, the use of radiation to mention

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