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Molecular Probes Based on Gold Nanoparticle

Author: JinYeZuo
Tutor: YanJiLin；TuYiFeng
School: Suzhou University
Course: Analytical Chemistry
Keywords: chemiluminescence gold nanoparticle aptamer restriction endonuclease colorimetric molecular recognition
CLC: TB383.1
Type: Master's thesis
Year: 2011
Downloads: 99
Quote: 0
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Abstract


In this paper, optical properties of gold nanoparticles are employed in the construction of probes for molecular recognition and sensing. Specific recognitions are achieved based on aptamer’s interaction with the target molecule and restriction enzyme’s with related substrate DNA strands. These probes provide simple, fast and sensitive molecules detection and the related procedure greatly is simplified.In the first part, the concept of probes, their composition and applications are reviewed, as well as the recognition systems and the signal reporting methods. In the final section, emphasis is focused on the role of gold nanoparticles, their characteristics and applications in the field of molecular probes.In the second part, we established aptamer sensing systems based on chemiluminescence resonance energy transfer. Gold nanoparticle acceptors and chemiluminescence donor groups are held together with aptamer linkages. Due to the close distance between the energy donor and acceptor, high efficient energy transfer occurs and the chemilumescence greatly quenched. With the presence of the target molecule, it is recognized by the aptamer and results in the break down of the linkage between the chemiluminescence group and gold nanoparticle, which enlarges the distance. A decrease of the energy transfer efficiency takes place and an enhancement of the chemiluminescence is obtained. Two types of probes are designed, one with covalent linked luminol for chemiluminescence while the other one is linking of a catalytic enzyme towards luminol chemiluminescence. Adenosine is adopted as a model analyte and the performances of the developed probes are studied.In the third part, we developed a simple colorimetric probe toward restriction endonuclease. Two nanoparticles are synthesized. One contains a specific palindromic sequence double strand DNA which can be recognized and hydrolyzed by the restriction endonuclease. After the hydrolysis, the residual single strand DNA hybridizes with a complementary sequence on the other particle, which leads to the aggregation of the two gold nanoparticles. Due to the change of particle size and related red-shift of the UV-vis spectrum from localized surface plasma resonance, a visible color change shows up. The designed probe provides an easy qualitative and semi-quantitative detection with no requirement of instrumentations. Also qualitative results are obtained with a UV-vis spectrometer.

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CLC: > Industrial Technology > General industrial technology > Materials science and engineering > Special structural materials
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