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Studies of Low-dose Ethanol Suppress Hepatocellular Carcinoma Cell Proliferation

Author: HuHui
Tutor: JingXuBin
School: Shantou University
Course: Digestion within the science
Keywords: Mitochondrial membrane potential Hepatocellular carcinoma Peroxide Ethanol Apoptosis
CLC: R735.7
Type: Master's thesis
Year: 2005
Downloads: 81
Quote: 0
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Abstract


Objective: To investigate the low concentration of ethanol in vitro inhibition of proliferation of hepatocellular carcinoma cell line SMMC-7721 and discussed the role of ethanol anti- hepatoma via . Method: 1) human hepatoma SMMC-7721 cells after resuscitation with RPMI1640 culture medium and culture at 37 ° C in 5% CO 2 < / sub > conditions until the cells covered flasks approximately 80% of the bottom of the bottle experiment. The experiment was divided into ethanol action group (experimental group) , saline treated group (control group) , ethanol and catalase role group ( pre-treatment group ) . Changes in cell morphology was observed under an inverted microscope . 2 ) ethanol role in SMMC-7721 cells by flow cytometry after intracellular H 2 O 2 level changes . Volume concentration of 2% , 4% , 6% ethanol acting on the cells , respectively, in the role of 1h after 2 hours , the flow cytometry mark cells H 2 O of the fluorescent dye, the fluorescence intensity of DCFH-DA . 3 ) the trypan blue exclusion assay ethanol to inhibit the proliferation of SMMC-7721 cells . Trypan blue staining to count , to detect the concentration of 4% ethanol effector cells 12h , 24h , 48h after the experimental group cells proliferation inhibition and inhibition of proliferation of cells with the same point in time preprocessing contrast . 4 ) ethanol role in SMMC-7721 cells by flow cytometry mitochondrial membrane potential changes . The experimental group and the control group , pretreatment of cells were role 3h , 6h , 12h , with the fluorescent dye DIOC mitochondrial membrane potential within the flow cytometry labeled cells 6 fluorescence intensity . 5 ) flow cytometry ethanol role in SMMC-7721 cell apoptosis peak . The experimental group and the control group , pretreatment of cells role 24h after 48 hours , the fluorescence intensity of the labeled cells DNA flow cytometry , fluorescent dye PI . Results: 1) under an inverted microscope to observe the morphological changes of SMMC-7721 cells . To the control group was observed under an inverted microscope fine

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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