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The Study on Rapid Detection of Bacterial in Burn Sepsis by 16SrRNA Gene Polymerase Chain Reaction

Author: WangXiaoPing
Tutor: ZuoDingHong
School: Jiangxi Medical College
Course: Surgery
Keywords: Burn Sepsis Blood culture Bacterial Universal primers 16SrRNA gene Polymerase chain reaction
CLC: R446.9
Type: Master's thesis
Year: 2005
Downloads: 74
Quote: 0
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Abstract


Objective: To early diagnosis of burn sepsis , discover a clinical rapid detection of bacterial infections . Methods: (1 ) collected according to certain standards of Jiangxi Medical College First Affiliated Hospital burn center hospitalization of 39 human peripheral blood samples of 41 patients with suspected sepsis . Each specimen is divided into two, respectively xue culture ( Blood Culture , BC ) and the universal primer and line 16S rRNA gene by polymerase chain reaction (Polymerase chain reaction PCR) detection bacteria , on analysis of the PCR product by agarose gel electrophoresis results , and the test results of PCR and BC both to analyze , compare the two methods in the detection of the burn sepsis bacterial pathogens on the speed and sensitivity . The establishment of a positive and negative control group, six cases in PCR experiments . ( 2 ) analysis of blood culture and sensitivity test results . Results: (1 ) using 16S rRNA gene PCR method detected 17 cases of PCR products expected 1.2kb long strip positive bacteria , 24 patients without bands appear negative bacteria . 6 patients with positive control specimens All the expected 1.2kb bands and 6 patients with negative control specimens were not there any bands . Blood culture positive for bacteria in 9 cases, negative 32 cases , eight cases of the PCR method was positive , the other cases PCR negative , positive rate of the 16S rRNA gene PCR method ( 41.16% ) blood culture positive rate ( 21.95% ) near twice , P lt ; 0.05 . Another PCR method for each batch of the results of only a single specimen of about 6 hours significantly higher than blood culture results To quickly take 12 to 24 hours . ( 2) 9 patients with blood culture positive patients , 8 cases of a single Pseudomonas aeruginosa infection , 1 case of single hemolytic staph infection . Susceptibility test report from the blood culture point of view , Pseudomonas aeruginosa clinical commonly used first - line broad-spectrum antibiotics generally resistant . Conclusions : ( 1 ) The 16S rRNA gene PCR detection of bacterial infections with rapidity and sensitivity advantages . (2) treated with antibiotics in the hospital burn center , causing the burn sepsis bacteria Pseudomonas aeruginosa . From blood culture susceptibility testing reports, the general resistance of Pseudomonas aeruginosa clinical commonly used first - line broad-spectrum antibiotics .

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