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[Objective ] The application of low doses of different concentrations of As 2 < / sub > O 3 < / sub > ( arsenic trioxide ) induced glioblastoma cell line BT325, through the changes in cell morphology , tumor differentiation markers expression changes in cell cycle phase distribution observed as 2 < / sub > the O 3 < / sub > -induced glioblastoma cell differentiation , induction of differentiation therapy for glioma theory basis . [ Method ] of BT325 cells As 2 O 3 -induced dose group ( 0 to 0.8úNPM ) three weeks after routine HE staining and electron microscopy cells induced morphological changes , immunocytochemical staining of three tumor differentiation marker GFAP ( glial fibrillary acidic protein ) , S - 100 ( S - 100 protein ) , vimentin ( vimentin ) expression changes , flow cytometry analysis of cell cycle phase distribution . [ Results ] of BT325 cells in arsenic trioxide for 3 weeks after inhibition of cell proliferation , the increase in size , abundant cytoplasm , nuclear -mass ratio decreased have obvious long thick synapses form close to the culture of normal astrocytes . Under the electron microscope the nucleus , cell membrane morphology rules organelle more normal . Immunocytochemistry 2 < / sub > O , As a 3 < / sub > of concentration is 0,0.1,0.2,0.4,0.8 úNPM- the average GFAP positive rate ( %) 8.09 ± 3.62,20.09 ± 6.21,40.51 ± 6.12,51.54 ± 7.27, 61.65 ± 10.85, S-100 average positive rate (%) 11.47 ± 4.87,22.71 ± 6.81,29.82 ± 5.82,46.64 ± 6.78,54.96 ± 15.82, GFAP, S-100 expression were increased , and showed a dose-dependent effect was significant difference ( P lt ; 0.05 ) between the experimental group and the control group ; of Vimentin average positive rate (%) 10.96 ± 5.66 , respectively , 7.56 ± 7.86,10.18 ± 5.33,9.13 ± 3.22,10.53 ± 4.92, no significant difference between the experimental group and the control group ( P gt ; 0.05 ) Flow cytometry , the concentration of As2O3 0,0.1,0.2,0.4,0.8 úNPM- the proportion of G0/G1 period are : 17.0 ± 2.00,21.3 ± 3.51,48.3 ± 10.40,51.0 ± 3.60 , 70.0 ± 2.00 , between the experimental group and the control group compare G 0 / the G 1 < / sub > of the proportion of significant difference ( P lt ; 0.05 ) , Tip
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