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Construction and Immune Reactivity of the Anti-tumor Vaccine of Recombinant Bacteriophage T7 Targeting Epidermal Growth Factor Receptor

Author: CaiXinHua
Tutor: ZhangQinXian；TanLiSong
School: Zhengzhou University
Course: Learning organizations learn and Embryology
Keywords: Epidermal Growth Factor Receptor T7 phage display Build Anti-cancer vaccine Antibody Cytotoxic T lymphocyte response
CLC: R392
Type: Master's thesis
Year: 2005
Downloads: 50
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Abstract

Cancer is a serious hazard to human purpose of health and disease , the lack of sensitive and effective treatment , often can not effectively curb tumor recurrence and metastasis. Therefore , the ideal treatment of explore urgent . Tumor biological treatment of low toxicity and low side effects have attracted widespread attention. Tumor immune therapy is one of the important biological therapy , at present, anti-idiotype antibody vaccine , vaccine antigens and DNA vaccines are all made ??some progress , but there are still some shortcomings , did not reach the desired results . Phage display technology (bacteriophage display) is exogenous protein or polypeptide as a fusion protein displayed on the phage capsid protein and the ability to maintain the original spatial structure and biological activity of widespread concern. Epidermal growth factor receptor (epidermal growth factor receptor, EGFR) is an earlier discovery of the tumor -associated antigen , epithelial malignancies have different degrees of high expression , the use of monoclonal antibodies for the treatment of malignant tumor EGFR expression has been some progress and enter clinical trials . The experiment was constructed recombinant EGFR as a target for bacteriophage T7 vector vaccines , and to further explore the immunological activity and in vivo anti-tumor effect of anti-tumor vaccine development foundation . Materials and methods by searching the GenBank database , according to the NCBI provides EGFR membrane outer part of the sequence , using the Primer Premier 5.0 software design primers by RT-PCR technology, from high expression of EGFR in human lung adenocarcinoma A549 cells cloned DNA EGFR outer membrane clips. Of 5 ' ends with EcoRI and HindIII restriction sites of primers , subcloned DNA fragment of about 1800bp ; with pMD18-T cloning vector , transformation, amplification , plasmids were extracted by the above Jikang Bioengineering Co., Ltd. sequencing. Bacteriophage T7 expression vector according to exogenous DNA fragment inserted length limits, and different regions of the outer membrane of EGFR features, using a protein analysis software , to obtain a better simulation of protein antigen presenting peptide sequences ; design each containing EcoRI and HindIII restriction sites primers were to identify the correct plasmid as a template , the DNA fragment was subcloned ; bacteriophage T7 expression vector and the arm link , packaging,

Construction and Immune Reactivity of the Anti-tumor Vaccine of Recombinant Bacteriophage T7 Targeting Epidermal Growth Factor Receptor

Abstract

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