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Objective: dragon's blood (Dragon's blood), also known as Kirin dried up , as commonly used in Chinese medicine blood stasis and hemostatic natured , sweet and salty . Compound Xuejie with other herbs with suffering . Gelable Xuejie insoluble in water , when exposed to heat , into glue when suffering is mixed with the rest of the dregs are filtered out , losing its pharmacological effects , thus wasting valuable resources of dragon's blood . In this study, using emulsion solvent evaporation method was Xuejie polylactic acid microspheres to achieve sustained action to reduce the number of medication , to provide a reference for the new formulation research and rational use of resources . Method : First, based on the 2000 edition of the Pharmacopoeia Xuejie application of thin-layer chromatography and ultraviolet spectrophotometry were identified and checked their rosin and alcohol insolubles , reversed-phase high-performance liquid chromatography determination Xuejie Dracorhodin content. Poly lactic acid as carrier, methylene chloride is an organic solvent , an aqueous gelatin solution as a dispersion medium , microspheres prepared with the emulsion solvent evaporation . The four factors as the nature of the selected impact microspheres more significant inspection object, i.e. , the concentration of the dosing ratio ( Xuejie : polylactic acid ), a dispersion medium (gelatin) , the organic phase, the concentration of the polylactic acid (polylactic acid mass : organic phase volume ) stirring speed , three levels of each factor , according to L9 ( 34 ) orthogonal design experiments arrangements to span ( uniformity ) , drug loading and entrapment efficiency indicators , comprehensive survey of influencing factors to determine the release microspheres ball prepared the optimum . Xuejie polylactic acid microsphere content was measured by reverse phase high performance liquid chromatography : select the appropriate detection wavelength suitable stationary phase and mobile phase composition , the ratio of flow rate peaks meet the testing requirements Dracorhodin adjust chromatography mobile phase so that the separation degree , the number of theoretical plates , symmetry factor required . And precision
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