|
Aquatic ornamental plants is an indispensable element to create water landscape and wetland landscape, but also to absorb the accumulation of heavy metal elements in water, water purification. Therefore, the use of aquatic ornamental plants to build artificial sewage treatment system, not only to achieve the purpose of decontamination, but also to create a beautiful water landscape. In this paper, the application in the field investigation on the basis of the three regions of Nanjing, Shanghai, Hangzhou, iris, canna, mosaic Arundo donax, yellow iris, ornamental value, such as eight kinds of aquatic ornamental plants as experimental materials to study the heavy metals in water pollution stress resistance. For the kinds of strong resistance (iris, canna) and a weak resistant species (mosaic Arundo donax) focuses on heavy metal defense key factor - the protective enzyme system, heavy metals by-products - reactive oxygen species the impact and the relationship between plants on the enrichment of heavy metals to explore the aquatic ornamental plants in the defense mechanisms of heavy metal pollution. And Analytic Hierarchy Process ornamental value, such as resistance, made a further comprehensive evaluation of these eight kinds of aquatic ornamental plants, three excellent aquatic elected new varieties of ornamental plants tissue culture techniques. The results of the study show that: (1) eight aquatic ornamental plants which, iris, water onion, canna heavy metals like mercury, cadmium has a strong resistance to mosaic Arundo donax weakest resistance of mercury ion contamination. Two heavy metal ions, the toxic effects of mercury ions on plants greater than cadmium ions. Three plants (iris, canna, mosaic Arundo donax) in the heavy metal pollution in the share of defense pathways: low concentrations of pollution, heavy metal ions induced reactive oxygen species start the defense reaction of the plant body, protective enzyme system can play an effective role in the resistance. Higher concentrations of pollution, heavy metal ions and unprotected enzyme system clears the combined effect of reactive oxygen species cause damage to the plant cell membrane, plant growth stops, and even death. Heavy metal pollution, three plants have the same defense mechanism: the plant through the roots of heavy metal ions bound to hinder the transport of heavy metals in plant, thus avoiding the heavy metal damage to the leaves and other organs; heavy metals in plant roots a large number of enrichment is considered to be a special mechanism of plant resistance to heavy metal poisoning. The eight aquatic ornamental plants evaluation results show that, canna, iris, yellow iris not only has a strong stress resistance, and the comprehensive evaluation is superior to other aquatic ornamental plants. On this basis, elected three new excellent aquatic ornamental plants: Mosaic iris, the gold leaf yellow iris, Canna, not only bright leaf, its cold resistance, stain resistance and the original species is not bad from top to bottom with great potential. 5 for the new superior aquatic ornamental plant propagation speed is slow, the market demand contradictions, the new superior aquatic ornamental plant tissue culture technology research. The main results are as follows: (1) mosaic iris adventitious buds, the sampling time of explant bud differentiation rate and proliferative effect of greater impact, drawn in spring is the best. MS medium supplemented with of 6-BA5mg / l NAA 0.5 mg / l is conducive to the differentiation and proliferation of adventitious buds per shoot proliferation around 6.0 times. In order to obtain a robust of rooted, before vaccination rooting, the first blank MS medium culture generation after rooting. The NAA mosaic iris plantlets rooting than IBA, a concentration of 0.5 mg / L, the highest rooting rate, rooting ideal. (2) golden yellow iris bud either inducing or pedicel induction, expressed in MS medium plus low concentration of 6-BA (1mg / l) and IBA0.1mg / l is the best average bud proliferation 2.85-fold. NAA has been shown to inhibit the differentiation and proliferation of adventitious buds, but the plantlets rooting experiment is conducive to the rooting of the plantlets. It was found that the glass of the plantlets
|