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Study on Agricultural Activity and Fungicidal Ingredient of Sophora Alopecuroides Linn. (Root)

Author: LiYanYan
Tutor: ZhangXing
School: Northwest University of Science and Technology
Course: Pesticides
Keywords: Sophora alopecuroides Activity was measured Separation and purification Active compounds Formulation Research
CLC: S482.292
Type: Master's thesis
Year: 2005
Downloads: 239
Quote: 2
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Abstract


Looking antibacterial substances from plant resources or lead compounds, based on the continuous development of the idea of ??the environmental harmony pesticides (Environment Acceptable Pesticides), development of Fungicide is an important way of current research and development of new pesticides. Preliminary studies show that the bitter beans (Sophora Alopecuroides Linn.) Root is its activity was higher in the site, and antimicrobial active ingredient present in the acetone, ethanol, water extract, bitter beans root alkaloids have higher antibacterial activity and medical value. Antimicrobial Activity of non-alkaloid constituents on the root of Sophora alopecuroides farm has not been reported, a more in-depth study in view of the author of the chemical composition of the root of the bitter beans Isolation and Antifungal Activities, and obtained the following results: 1. The solvent extract of bitter beans root powder parallel cold soak test to determine the ethanol extraction solvent is more appropriate. Through the root system pre-trial of the ethanol extract phytochemical round filter paper method bitter beans that bitter beans contain steroidal, terpenoids, flavonoids and their glycosides, amino acids, alkaloids, esters, Coumadin somatomedins, anthraquinone and its glycosides, reducing sugars, volatile oils, such as chemical composition. Tested the bitter beans root crude extracts sterilization, weeding and antiviral activity, the results show that: (1) bitter beans root powder samples tested concentration 0.5mg/mL ethanol extract of Phytophthora capsici Rice sheath blight fungus, Sclerotinia sclerotiorum, Exserohilum turcicum, Botrytis cinerea, Apple anthrax bacteria has good in vitro mycelial growth inhibition the basic inhibition rate reached more than 50%. (2) bitter beans root the ethanol extract extract its part (0.02g/ml) have a certain resistance to TMV virus activity, the highest activity of ethyl acetate segment, passivation effect of 96.03%, followed by ether segment 75.625% 54.26% ethanol extract of 45.07% of the chloroform segment. (3) bitter the beans root ethanol extract (for the test sample concentration of dry sample 0.2g/ml) young roots and shoots of grass seed redroot pigweed, alfalfa, wild oats, perennial ryegrass heterozygous (animal husbandry) growth have a better inhibition, over 60% (except for alfalfa shoots); the Amaranthus retroflexus strongest inhibition is almost 100%. 3 test the bactericidal activity of the compounds K2, K3, and K4. (1) determination of the compounds K2, K3, K4 (tested concentration of 0.05 mg / ml) of Phytophthora capsici, Rhizoctonia, Apple anthrax bacteria and tomato Botrytis cinerea in vitro mycelial growth inhibition, showed that: compounds K3 basic four pathogens have good activity, a minimum of 45.45% up to 95.00%; the compound K2 antibacterial effect is relatively lower: three compounds in general have good antibacterial effect of Botrytis cinerea were higher than 65%: the compound K4 Phytophthora capsici best antibacterial effect relative of 57.27%. (2) fruit needling test compounds K2, K3, K4 (tested concentration 0.5mg/ml) in vivo inhibition of Botrytis cinerea, the results show that the protection and treatment of the role of the three tomato gray mold than those , more than 60%, and its protective effects are better than the treatment effect; K3 Bacteriostasis effect best protective and therapeutic effect higher than 80%: K2 on tomato gray mold fruit stronger than its off body bioassay results, K3, K4 in vitro bioassay results better than in vivo.

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CLC: > Agricultural Sciences > Plant Protection > Pesticide ( chemical control ) > Various pesticides > Antiseptic > Biological sources of fungicides
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