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The Enhancement of Irradiation to Hypoxic Human Cervical Cancer Line(Hela Cell) by Hyperthemia and CMNA

Author: YinJinJun
Tutor: LiuHuiZhong
School: Shandong University
Course: Oncology
Keywords: HeLa cells Glycodidazolum sodium Hyperthermia SER OER TER
CLC: R737.33
Type: Master's thesis
Year: 2005
Downloads: 78
Quote: 0
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Abstract


Objective To observe the hyperthermia and sodium glycodidazolum the human cervical enhanced role , lack of oxygen in HeLa cells radiation therapy to explore a reasonable lack of oxygen sensitizer mode . Method 1 . Establish hypoxic HeLa cell model for hypoxic cells irradiated group for the test group , aerobic cells irradiated group as a control group , count the number of clones vitro cell colony formation method , fitting cell survival curve , different situations , a D 0 values ??to calculate the OER . Conducted of sodium of glycodidazolum toxicity test . Vitro cell colony formation method for vertical and horizontal coordinates were CMNa concentration and cell viability draw scatterplot , curve fitting to SAS8.0 , linear equation , calculated ID 50 and its non-toxic concentration . 3. Lack the oxygen irradiation 0.24mmol / l , 0.48 mmol / l , 1.44 mmol / l glycodidazolum sodium for the test group for the control group , the hypoxic exposure alone vitro cell colony formation method to count the number of clones obtained was under different circumstances D 0 values ??fit the cell survival curve , calculate their respective SER . 4 . Aerobic cells in vitro cell colony formation method to compare with the thermal sensitivity of hypoxic cells . 5. Before irradiation , hyperthermia, hyperthermia after irradiation for the experimental group , the simple irradiation for the control group were given different doses of X-ray irradiation vitro cell colony formation method to count the number of clones , fitting cell survival curve , calculated each TER. 6. Elect optimized combination : the the irradiation high concentration of glycyl of Sodium hyperthermia ( after radiotherapy ) with radiation alone as the control , curve fitting , calculate the SER . Results . Hypoxia model successfully established the the calculated D 0 aerobics was 1.21 , D hypoxia to 3.25 OER 2.76,2 with different concentrations of CMNa as the independent variable x, and cell survival as a result of the variable Y is plotted scattergram , found that with the increase of the concentration of the independent variable , due to the value of the variable cell viability acceleration decreasing trend , it can be chosen exponential curve for curve fitting . Take y = lgY, curve fitting after SAS8.0 , linear equation lgY =-0.0952-0.0099x, that is Y =

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
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