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Study on Developing and Application of Herbicide-resistant Rice(Oryza Sativa L)

Author: WuHuiMin
Tutor: MouTongMin；LinYongJun
School: Huazhong Agricultural University
Course: Crop Genetics and Breeding
Keywords: Herbicide Resistant Rice Transgenic Agrobacterium-mediated transformation bar gene CP4-EPSPS gene Hybrid purity
CLC: S511
Type: Master's thesis
Year: 2005
Downloads: 268
Quote: 6
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Abstract

Rice (Oryza sativa L) is the major food crops in China and the world, is the main source of calories for nearly half of the population. In China, the first of its total area, output and yield per unit area tops in various types of food crops. National rice yield losses due to weeds directly caused more than ten million tons, controlling weeds not only the input of manpower, material and financial resources, and also sprayed a large number of herbicides, causing serious pollution to the environment. With herbicide-resistant genetically modified soybeans, corn, rapeseed production on large-scale cultivation, resulting corresponding substantial reduction in the use of herbicides and increasingly demonstrate the superiority of the herbicide-resistant crops. Therefore, the use of plant genetic engineering herbicide-resistant high-quality high-yielding rice varieties, cost-effective control weeds, thus promoting China's grain production. The study by Agrobacterium-mediated transformation will herbicide glufosinate resistant grass phosphinothricin acetyltransferase gene (bar gene) synthesis and artificial modification with the herbicide glyphosate resistance CP ₄-EPSPS gene into rice varieties, glufosinate herbicide-resistant and herbicide glyphosate transgenic plants, the use of existing herbicide-resistant rice varieties hybridization turn sterile and anti weeding the agent Rice feasibility of the application to the actual production was discussed. Its main findings are as follows: 1 through Agrobacterium tumefaciens-mediated method will the herbicide glufosinate resistance gene bar gene and artificial modification of synthetic herbicides glyphosate resistance gene of the CP ₄ -EPSPS genes into rice varieties 2354, Minghui 63 and 11 spent. Which rice varieties, 2354 a total of 30 transgenic plants, Minghui 63 of 45, spent 11 207. 2 PCR, the Field Resistance, and Southern blot hybridization assay: herbicide resistance bar gene has been integrated into the rice genome, and resistant plants, mostly single copy insertion. PCR and herbicide resistance test showed: by Agrobacterium-mediated CP ₄-EPSPS gene was integrated into the rice genome, the artificial modification synthesis CP ₄ EPSPS The gene has a resistance to the herbicide glyphosate. The results show that for the first time the use of glyphosate cultivate glyphosate-resistant rice to be successful as a screening agent. 3 herbicide-resistant new line with the original varieties of agronomic traits by backcrossing has bred the UOB 25 and Minghui 63 has a new line of herbicide resistance. 4 different concentrations of herbicides and different periods of treatment against herbicide rice growth impact study showed that seedlings 3-5 leaf stage with 15% of the Basta diluted 600-800X to remove false hybrids to improve the purity of the hybrid field, the best results; The rice planting tillering with 600-800X the Basta herbicide spraying, weeds growing, and to improve rice yield can be effectively controlled. The 5 different herbicide treatment research to improve the purity of the hybrids effects 3-leaf stage in the seedling spraying 600X15? Sta herbicides hybrid field can be 100% purity, confirmed the herbicide resistance gene used to improve the hybrid system kinds purity feasibility.

Study on Developing and Application of Herbicide-resistant Rice(Oryza Sativa L)

Abstract

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