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Adsorption-Desorption, Nuclease Degradation and Microcalorimetric Characteristics of DNA on Red Soil Active Particles

Author: ZhangXueWen
Tutor: HuangQiaoYun
School: Huazhong Agricultural University
Course: Soil
Keywords: DNA Soil colloids Mineral Adsorption Desorption Nuclease Degradation Microcalorimetry Enthalpy change
CLC: S154.2
Type: Master's thesis
Year: 2005
Downloads: 151
Quote: 0
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Abstract


Xianning Spa red soil is divided into 0.2-2μm organic clays ,0.2-2μm inorganic clays less than cosmids the 0.2μm containing organic matter and clay less than 0.2μm go organic matter. The red soil colloids, kaolinite and goethite as experimental materials to study the adsorption and desorption of the DNA in the soil colloids and mineral surfaces, degradation of DNA adsorption microcalorimetry characteristics. The main results are as follows: 1. System distinguish the variable charge red soil activity particles on DNA adsorption. Kaolinite and less than 0.2μm clay (fine clay) controls the amount of DNA adsorption, the larger contribution of the organic matter on the adsorption of DNA, goethite small contribution. DNA and 0.2-2μm the cosmid (rough clay), go organic matter between cosmid and kaolinite, affinity, with fine clays containing the smaller affinity between organic clays and goethite. The soil conditions have a significant impact on DNA adsorption. The system pH increased from 2.0 to 5.0, the DNA in the soil colloids and mineral surface adsorption significantly reduced; increase in pH from 5.0 to 7.0, the adsorption process is a slow decrease. The electrolyte MgCl more than NaCl promote the adsorption of the DNA in the soil colloids and mineral surfaces. 3 through the sodium acetate buffer, sodium chloride and sodium phosphate buffer, serials desorption soil colloids and mineral surfaces DNA adsorbed proportion of the DNA to various forces. DNA in a lower proportion of red soil colloids and mineral surface adsorption through electrostatic interactions, only 10-20%. Organic clays and kaolinite, DNA may be mainly through dehydration and hydrogen bonding, inorganic clays and goethite surface, DNA, mainly through ligand exchange adsorption, accounting for 43.3-65.2%. 4 the application gel electrophoresis technology, for the first time reported that the DNA is not fixed strength degradation of DNA in the red soil colloids and mineral surfaces, mainly depends on soil colloids and mineral surfaces nuclease activity. In the variable charge red soil, organic matter and particle size had no significant effect on the degradation of DNA. 5. Calorimetry for the first time, determined by DNA in soil colloids and mineral surface adsorption thermodynamic parameters for the DNA in the particle surface adsorption mechanism of soil active clarifies the new evidence. DNA in the red soil colloid or mineral surface adsorption is a process of entropy increase. The adsorption of the DNA in the organic clays and mineral surfaces accompanied by endothermic reactions, which may be due to the destruction of the old hydrogen needed more energy, as well as a common result of electrostatic repulsion and dewatering. DNA in the cosmid the adsorption of organic matter is an exothermic reaction, which may be the result of electrostatic attraction between the DNA and inorganic clays and ligand exchange interaction. With MgCl 2 the increase of the concentration and pH decreases DNA in kaolinite and goethite surface adsorption enthalpy gradually reduce. In the high-pH and low-MgCl 2 concentration, adsorption

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CLC: > Agricultural Sciences > Agriculture as the foundation of science > Soil > Soil Biology > Soil biochemistry
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