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Effects of Soybean Oil on the Fermentation of GA3 Produced by Gibberella Fujikuroi 978

Author: WangJiGang
Tutor: ChenShouWen
School: Huazhong Agricultural University
Course: Microbiology
Keywords: Fujikura Gibberella Gibberellin GA3 Ferment Bean oil Up the oil in batches fermentation The activity of lipase
CLC: TQ452
Type: Master's thesis
Year: 2005
Downloads: 110
Quote: 3
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Abstract


In order to improve the fermentation of the gibberellin GA3 production strains Fujikura Gibberella (Gibberella fujikuroi) 978 strains production levels, GA3 fermentation leading carbon source of starch fermentation process based on soybean oil in different ways GA3 fermentation impact, initial exploration GA3 titer. Starch as the dominant carbon source the gibberellin GA3 complement oil fermentation experiments showed that the added concentration of soybean oil and add time to GA3 fermentation titer, and fermentation storage of Gibberella use add soybean oil case, the added soybean oil to improve the potency of GA3 help. The the starch fermentation medium fermentation 60h add 1.0% (W / V) of soybean oil, fermented 192h, the GA3 titer starch fermentation medium fermentation compared to a 21% increase in shake flask fermentation level, 20 in 10L fermentor levels improve %. Soybean oil can be added portionwise to avoid inhibition of the ability of dissolved oxygen in fermentation broth is also important, but the time and concentration of soybean oil added, the starch fermentation medium fermented 60h and 108h each adding 0.5% (W / V) of soybean oil, fermentation 192h, GA3 production of starch fermentation medium fermentation compared the flask fermentation level by 26% and 29% in 10L fermentor levels improve. Soybean oil as the dominant carbon source gibberellin GA3 fermentation experiments showed that the the concentration be fermented soybean oil solution dissolved oxygen (DO value) impact, thereby affecting the Fusarium biomass and GA3 potency. The medium optimization results: soybean oil concentration of 6% (W / V), the nitrogen source is 1.2% peanut meal, adding 0.001mol/LMnSO 4 and 0.1% polyethylene glycol (PEG) The other ingredients with starch fermentation medium, fermentation in shake flask fermentation level 192h the GA3 potency relative to the 32% increase in starch fermentation medium fermentation; 180h in 10L fermentor level of fermentation, the titer increased by 30%. Fermentation experiment showed that the portionwise additional soybean oil, in a reasonable additional time and the concentration of soybean oil under the condition will be the ideal GA3 titer. 3% (W / V) of soybean oil for the fermentation starting dominant carbon sources, supplemented with 2% (W / V) of soybean oil and fermentation 108h supplemented with 1% (W / V) of soybean oil, fermentation 192h, GA3 titer in the fermentation 60h compared with starch fermentation medium fermentation flask fermentation level increased by about 38%. However, soybean oil additional mechanism is necessary to carry out in-depth research. Phased provide different dominant carbon source gibberellin GA3 fermentation experiments show that using the fermented starch as a starting carbon source, while adding the right amount of soybean oil pre-fermentation induced lipase enzyme fermentation broth of residual sugar and fat additional soybean oil change may guarantee Gibberella biomass and raise the titer of the GA3, while avoiding the end of fermentation of soybean oil residue. The optimized fermentation ways: 4% corn starch as a fermentation starting dominant carbon source, a reducing sugar concentration of about 22g / L, while adding 1% (W / V) of soybean oil, fermentation 60h when supplemented with 4% (W / V ) soybean oil, fermented 192h, fermentation compared to a 32% increase in the the flask fermentation level, GA3 potency starch fermentation medium and 38% in 10L fermentor levels improve. In experimental batches supplemented with soybean oil, soybean oil supplemented with additional storage period should GA3 fermentation time was appropriate to take advantage of. Fermentation 60h and 108h supplemented with 2% (W / V) of soybean oil, fermented 192h, the flask fermentation level GA3 yield more starch fermentation medium fermentation to improve by 40%.

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