Dissertation > Excellent graduate degree dissertation topics show

Research on in Vitro Embryo Production in Sheep

Author: ZhengXiaoMin
Tutor: ShiYuanGang
School: Ningxia University
Course: Clinical Veterinary Medicine
Keywords: Oocytes In vitro fertilization Sheep Embryos
CLC: S826.3
Type: Master's thesis
Year: 2005
Downloads: 79
Quote: 1
Read: Download Dissertation

Abstract


IVF, in vitro embryo production technology has been assisted breeding research focus of the new technology. Sheep in vitro fertilization technology system is not very mature, the experimental use of slaughterhouse sheep ovarian aims to study sheep in vitro maturation, in vitro fertilization (IVM / IVF) and early embryo culture influencing factors, in order to filter out the more reasonable sheep oocytes in vitro maturation, in vitro fertilization and early embryo development culture system. The results show that: 1 cut sectional slaughter sheep ovarian surface follicles collection available oocytes significantly more than aspiration (4.47 / ovarian VS2.51 gold / ovarian), but it takes a long time (55 seconds vs51 seconds ). Add 2 different doses of FSH can stimulate oocyte in vitro maturation, and among the groups of the oocyte maturation rate did not significantly different (P gt; 0.05); However, adding 1μg/ml and 5μg/mlFSH of maturation medium the cleavage rate was significantly greater than to add the the maturation medium 10μg/ml FSH (P lt; 0.05). 3 shows that sheep mature culture was added dose of EGF can significantly increase the rate of mature oocytes and 100ng/ml of EGF to add the the concentration 0ng/mlEGF and 50ng/mlEGF add concentration the comparison also significantly improve after fertilization sheep eggs cleavage rate of the mother cell, it is recommended that a suitable addition concentration. 4 culture medium granule cells within a certain concentration range (concentration of 2-6 × 10 ~ 6 / ml), no significant effect on the presence of oocyte maturation rate, but it can significantly improve the oocytes fertilized eggs split rate (55.6% vs53.8%) and mulberry sac rate (28.9% vs19.1%) (P lt; 0.05). 5 the Rams individual impact on IVF results can not be ignored. The experimental results show that, from three different ram semen by the same capacitation, oocyte IVF results in cleavage rate (54.7%, 41.3%, 43.3%) and morula embryo / blastocyst rate ( 28.2%, 19.7%, 22.6%) have a significant difference (P lt; 0.05) 6 sheep fertilization cleavage rate of 16 hours, mulberry sac was significantly higher than the fertilized 8 hours, 12 hours and 20 hours. The 7 four culture medium Ⅰ liquid: TCM199 GCM 15% ESS EGF; Ⅱ solution: TCM199 15% ESS EGF; Ⅲ solution: SOF liquid of of 15 ESS 10μg/ml NEAA 20μg/mlEAA of EGF; IV fluid: SOF liquid BSA 10μg/mlNEAA 20μg/mlEAA EGF co-cultured embryos after fertilization Ⅰ, II, III solution cleavage rate was significantly greater than group Ⅳ culture medium (P lt; 0.05), Ⅰ Yexian greater than Ⅱ liquid (P lt; 0.05 ) III liquid and Ⅰ, Ⅱ liquid, the difference was not significant. Four groups of embryonic development culture fluid obtained between mulberry sac rate Ⅰ solution higher but with the other three groups differences not statistically significant (P gt; 0.05). The of 8 sheep oocytes in vitro maturation, in vitro fertilization and early embryo culture TCM199 IVF gradient culture system compared with conventional in vitro fertilization system currently in use, the maturation rate difference was not significant (81.3% vs80.4%), but oocytes cleavage rate after in vitro fertilization (72.1% vs50.8) and Sang sac rate (94.8% vs24.5%) difference was very significant (P lt; 0.01).

Related Dissertations

  1. Cloning, Expression and Polymorphism Analysis of BMP4 Gene in Hu Sheep,S826
  2. Functional Analysis of a Phosphate Transporter Gene OsPtl in Rice,S511
  3. The Effects of Isoflavones in Soybean Straw on the Growth and meat Quality of Goat,S826.5
  4. Vitrification of Porcine Early Stage Embryos Reconstructed by Somatic Cell Nuclear Transfer,S828
  5. Study on Molecular Markers of the Prolificacy in Hu Sheep,S826
  6. Development of the Colloidal Gold Immuno-Chromatographyic Strips for the Early Pregnancy Diagnosis of Hu Sheep,S858.26
  7. Study on the Relationship between the Reproductive Ability of Hu Sheep and the Expression Level of Genes Correlated with Ovulation,S826
  8. Effects of Cumulus Cells on Maturarion in Vitro and Subsequent Developmental Competence of Porcine Oocytes,S828
  9. Effects and Mechanisms of Action of Restraint Stress on Oocyte Developmental Potential in the Mouswe,S865.13
  10. Seroepidemiological Survery of Hepatitis E from Sheep in Xinjiang and Cloning and Expression of HEV ORF2-62 Gene,S858.26
  11. Study on the Techniques in Bovine Oocytes Freezing and Parthenogenesis in Vitro,S823
  12. AFLP Analysis of Genetic Polymorphismin Kazak、Aletai Bashibai of Indigenous Sheep Breeds in Xinjiang,S826
  13. The Cloning and Sequence Analysis on actA、inlB and Distribution in Artificial Infectious Mice Disease Model for Listerial Monocytogenes,Q78
  14. Study on the Effect of Cotton Meal and Cottonseed Shell on Urolithiasis of Male Fine Wool Sheep,S858.26
  15. Study of Laparoscopic Abomasal Fistula Surgery in Sheep,S858.26
  16. Study of Laparoscopic Biliary Drainage in Sheep,S858.26
  17. Superovulation of Lambs and Its Fertilization in Vitro,S826
  18. Mterfd2 expression and regulation mechanism and the preliminary study on the biological function,Q78
  19. The Expression of HOXA10 and Leukemia Inhibitory Factor in the Endometrium of Patients with Hydrosalpinx and Its Correlation with the Outcome of IVF-ET,R714.8
  20. Establishment of Rapid PCR and Application of Sex Determination of Sperm and Embryo of Bovine,S823
  21. Preparation of the Multiple Epitopes Vaccine Against Foot-and-mouth Disease Virus Type Asia 1 in Sheep,S852.5

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Sheep
© 2012 www.DissertationTopic.Net  Mobile