Dissertation > Excellent graduate degree dissertation topics show

Study on Regeneration System from Leaf in Vitro and Protoplast Isolation and Culture of Black Chokeberry

Author: ZhangChunYu
Tutor: ZhangZhiDong
School: Jilin Agricultural University
Course: Pomology
Keywords: Black fruit gland rib Sorbus Leaf Regeneration System Suspension Protoplasts
CLC: S663.9
Type: Master's thesis
Year: 2005
Downloads: 249
Quote: 2
Read: Download Dissertation

Abstract

Black fruit gland rib Sorbus (Aronia melanocarpa) is an emerging small berry fruit. In large areas of the world have cultivated, but the area under cultivation in our country is very small. Because black fruit gland rib Sorbus dominant gene of value restrictions, making the means of improvement through conventional breeding cultivars severely limited. It has been shown, the use of biological techniques for gene transfer and somatic cell fusion can be overcome by the nature of the species formed in reproductive isolation for germplasm innovation provides a novel and effective way. The trial was the first rib with black fruit rowan gland as material for a detached leaf callus induction, regeneration system, the cell suspension culture, protoplast isolation, cell culture and protoplast culture, as well as factors related to the research aimed at establishing black fruit rowan leaf rib gland regeneration system and cell suspensions obtained single cells and protoplasts regenerated for future black fruit of Sorbus gland rib somatic hybridization and genetic transformation lay the foundation. 1 black fruit rowan gland rib callus induction from leaf to leaf as explants for callus induction test results showed that: plant growth regulator type and concentration of the black fruit of Sorbus callus gland rib type and growing volume has a great influence. Cytokinin BA and CPPU alone did not induce callus formation, alone auxin NAA and 2,4-D or auxin (NAA ,2,4-D and IBA) and cytokinin (BA, CPPU, ZT) can be used in conjunction with both callus formation. In MS 2,4-D 1.0mg / L, 3000Lux light intensity during the day and 25 ℃, the night 20 ℃, 16h light, 8h dark, pH6.0 and sucrose content of 3% for the most suitable callus culture conditions, the fresh weight and dry weight increased the most. Only 2,4-D callus is loose type, other hormones and their combinations are compact callus type. 2 blackbody gland rib Sorbus Leaves regeneration system is affecting plant growth regulator Regeneration System leaves a major factor. Studies show that: auxin and cytokinin concentrations and combinations on leaf Redifferentiation influence. NAA favor differentiation roots, when the concentration of NAA 0.5mg / L when the rooting rate of up to 90%. Cytokinin and auxin used in conjunction benefit buds. In step was induced in MS IBA1.0mg / L BA3.0mg / L when the induction rate was 50%, but the average number per leaf bud differentiation is only 2. In the two-step method of inducing the formation of callus using WPM CPPU2.0mg / L 2,4-D0 .1 mg / L of culture medium, transferred to callus differentiation medium WPM CPPU2.0mg / L, the regeneration rate reached 93.3%, and the average number of regenerated shoots 15.2. Using the two-step method to establish an efficient induction of regeneration system. 3 black fruit gland cell suspensions rib Sorbus establish 2,4-D induced white loose callus cell suspensions can be established, and the MS BA2.0mg / L NAA0.5mg / L induced by dense green Callus cell suspensions can not be established. MS basic medium using superior B5, WPM, AA and N6 medium; in MS 2,4-D 1.0mg / L medium supplemented

Related Dissertations

CLC: > Agricultural Sciences > Gardening > Fruit trees gardening > Berries > Other
© 2012 www.DissertationTopic.Net Mobile