Dissertation > Excellent graduate degree dissertation topics show

The Study of Expression of Myeloid-related Protein-8 and -14, CD11b and MMP-9 in Kawasaki Disease

Author: FanQiuHong
Tutor: YanWenHua
School: Suzhou University
Course: Pediatrics
Keywords: Kawasaki disease (KD) MRP8/MRP14 Neutrophil adhesion molecule CD11b Matrix metalloproteinase -9(MMP-9) Coronary arterial lesion (CAL)
CLC: R725.4
Type: Master's thesis
Year: 2011
Downloads: 27
Quote: 0
Read: Download Dissertation

Abstract


Objective:In order to investigate pathogenesis of vasculitis and CAL in Kawasaki disease (KD) and sought to laboratory diagnostic criteria and new targets for drug therapy for KD.we performed this study focusing on the Plasma MRP-8/MRP-14 heterodimers (Myeloid- Related Protein-8 and-14) and matrix metalloproteinase-9 (MMP-9) as well as MRP8mRNA, MRP14mRNA and Neutrphil adhesion molecule CD11bmRNA in Leukocyte in KD, to observe the change expression of MRP-8/MRP-14 heterodimers and Neutrphil adhesion molecule CD11b and matrix metalloproteinase -9 in KD.Methods:According to the criteria set in 2002 in Japan, 46 KD inpatients from July 2009 to December 2010 were objects of study. 25 febrile children with infection formed febrile groups. 20 healthy children formed health control groups. Refered to echocardiogram diagnostic standard, samples from KD patients were divided into two groups:coronary artery dilatation (CAD) groups (n=15) and cornary artery normal groups (n=31). Collecting peripheral venous blood in the actue and subacute stage of KD. MRP- 8/MRP- 14 and MMP-9 in Plasma were both detected with enzyme linked immunosorbent assay (ELISA). Gene expression of MRP-8, MRP-14 and CD11b in Leukocyte was analyzed by Semi-quantitative reverse transcription–polymerase chain reaction (RT-PCR).Result:1. The white blood cell count, neutrophil percentage and C-reactive protein were significantly higher in the acute KD than the sub-acute phase (P <0.0001).2. The plasma MRP-8/MRP-14 levels as well as mRNA expressions of MRP-8 and -14, CD11b in Leukocyte were significant higher in patients with acute KD than in febrile and healthy control patients (P<0.001), and decreased dramatically within subacute stage (P<0.001). But still higher than the febrile group and the healthy control group (P<0.05).3. The plasma MMP-9 level was significant higher in patients with acute KD than in febrile and healthy control patients (P<0.05), and decreased dramatically within subacute Stage (P<0.001). And no different from febrile and healthy control patients.4. The plasma MRP-8/MRP-14 and MMP-9 as well as mRNA expressions of MRP-8 and -14, CD11b in Leukocyte were significant higher in KD with coronary artery dilatation than in coronary artery normal patients (P<0.05).5. MRP-8/MRP-14 was correlated with MMP-9 in the acute phase of KD patients (r=0.503, P=0.00). MRP-8/MRP-14 was correlated with CD11bmRNA in the acute phase of KD patients (r=0.782, P=0.00). MMP-9 was correlated with CD11bmRNA in the acute phase of KD patients (r=0.540, P=0.00).6. MRP-8/-14 was correlated with white blood cell count and neutrophil percentage in the acute phase of KD patients (r=0.483, P=0.001; r=0.310, P=0.036). MRP-8/MRP-14 was not correlated with Platelet (r=0.059, P=0.697). MMP-9 was correlated with white blo-od cell count and neutrophil percentage in the acute phase of KD patients (r=0.598, P=0.00; r=0.453, P=0.02). CD11b was correlated with white blood cell count and neutrophil percentage in the acute phase of KD patients (r=0.404, P=0.005; r=0.367, P=0.012).Conclusion:1. MRP-8/MRP-14, Neutrophil adhesion molecule CD11b and MMP-9 may probably play a role in the pathogenesis of vasculitis and can be used as a reference indicator in KD.2. MRP8/MRP14, Neutrophil adhesion molecule CD11b and MMP-9 were correlated with each other in KD. they together participate in KD inflammation, especially endothelia lesion and platelet activation. And they may be involved in the formation of CAL. They can be used as one of the indicators to predict the CAL formation.3. MRP8/MRP14 and CD11bmRNA were still higher than the febrile group and the healthy control group in the subacute of KD, indicating the persistence of inflammation and endothelial microvascular injury. This suggested that anti-inflammation of anticoagulation therapy remains need in the subacute of KD.4. MRP-8/MRP14, MMP-9 and CD11b were correlated with white blood cell count and neutrophil percentage in the acute phase of KD patients.It demonstrated that they were closely related with acute inflammation activity of KD. They are closely connected the severity of inflammation.

Related Dissertations

  1. Risk Factors Evaluation of Intravenous Immunoglobulin -Nonresponsive Kawasaki Diseas,R725.4
  2. An Experimental Research of the Influence of CO2 Environment on the Biological Specificity of Human Colon Carcinoma,R735.35
  3. The Correlation of the Expression of CD11b/CD18 on Neutrophils in Peripheral Blood and the Concentrations of IL-8 Serum in COPD,R563.9
  4. Role of TLR4 Signaling Pathyway in Pancreatic Adenocarcinoma Cells,R735.9
  5. Experimental Study of Molecular Pathogenesis of Hepatocyte Carcinoma Metastasis Mediated by P120ctn,R735.7
  6. An Experimental Study on the Effects of MAPKs Signal Pathway in Espressing MMP-1/-13 of Rabbit Osteoarthritis Chondrocytes,R684.3
  7. The Effect of eNOS、MMP-1 and TIMP-1 in the Pathogenesis of Pulmonary Hypertension Associated with Congenital Heart Disease,R725.4
  8. A Preliminary Study on the Effect of Lentivirus-mediated Gene Silencing of Interleukin-1 Type Ⅰ Receptor in Osteoarthritic Rats,R684.3
  9. The Effect of Allicin on Bleomycin-induced Pulmonary Fibrosis Rats,R285.5
  10. The Effect of Xien Yu Tang on Treating Sensitized Mice Against Lung Cancer Cells and the Study on Expression of MMP-2 and TIMP-2,R273
  11. The Relativity between MMP-2,Fibronectin with Metastasis in Breast Cancer,R737.9
  12. Study on Mechanism of VEGF Increasing Permeability of Endothelial Cells to LDL,R363
  13. Study of the mechanisms on the modification of extracellular matrix during orthodontic periodontal tissue remodelling,R783.5
  14. Extracellular matrix degradation system in the pathogenesis of polymyositis rats,R746
  15. Investigation on the Inhibitory Effect of Recombinant Polypeptide CH50 of Fibronectin on Invasion and Angiogenesis of Tumor,R730.5
  16. The Prophylactic Effect of Retinoic Acid on Elastase-induced Emphysema in Rats,R563.3
  17. The Expression and Significance of Matrix Metalloproteinase-9 in Human Lupus Nephritis,R593.242
  18. The Expression of CD44v6 and MMP-2 in Extrahepatic Cholangiocarcinoma and Their Clinical Significance,R735.8
  19. And selective inhibitor of cyclooxygenase-2 in gastric cancer tissues CD44V6, MMP-9 expression,R735.2
  20. Experimental Study on the Effect of Statins on Matrix Metalloproteinase-1 (MMP-1) and Matrix Metalloproteinase Tissue Inhibitor-1(TIMP-1) Secreted by Rat Macrophage,R96
  21. Correlation Study between the 16-slices CT Dynamic Enhancement and Microvessel Density、VEGF、MMP-2 Expression in Determining Invasion and Metastasis of Laryngeal Carcinoma,R739.6

CLC: > Medicine, health > Pediatrics > Children within the science > Pediatric cardiac and vascular disease
© 2012 www.DissertationTopic.Net  Mobile