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Expression of Cyclin D1 and p27 at Different Stage of Human Dermal Hemangiomas

Author: ShenPeng
Tutor: ShanShengGuo
School: Wuhan University
Course: Surgery
Keywords: Skin Hemangioma cyclin D1 gene p27 gene
CLC: R739.5
Type: Master's thesis
Year: 2005
Downloads: 30
Quote: 0
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Abstract


Hemangioma is a common benign tumors occur in the infant. Endothelial cell proliferation and the rapid growth of blood vessels is the most prominent feature of the histopathology of hemangioma hemangioma occurs in real terms. Endothelial growth intake to 3H-thymidine, mast cells, fibroblasts formed and fatty infiltration hemangioma is divided into three periods: the proliferative phase, degradation and degradation of completion of, but so far the exact mechanism of the pathological evolution still not very clear. In recent years, studies have shown that the hemangioma is a vascular excessive formation of benign tumors, excessive proliferation of vascular endothelial cells and angiogenesis plays a very important role in the the hemangioma pathology evolution, and the impact of the excessive proliferation of vascular endothelial cells and vascular The study of the formation of factors has drawn increasing attention of researchers. Objective: To investigate cyclin D1 and p27 protein in hemangiomas occur in the expression and its significance in the process of development and degradation. Methods: This study collected complete clinical and pathological data of 49 patients with cutaneous hemangioma surgical specimens, routine HE staining and immunohistochemistry SP method proliferating cell nuclear antigen (PCNA, proliferating Cell Nuclear Antigen) the Mulliken classification criteria and combined with PCNA expression of staging; then detected 49 cases of human skin hemangioma proliferative phase, degradation and 5 cases of normal skin tissue cyclin D1 and p27 expression level by immunohistochemistry (SP method), and factor Ⅷ related antigen Immunohistochemical staining confirmed the expression of cyclin D1 and p27 cell vascular endothelial cells; multimedia test results obtained application HPIAS-2000 color pathological image analysis system (Tongji 1000 screen imaging company) for processing and analysis of the tissue chemical reaction positive particles the average optical density and average positive area ratio. Results: 1 hemangioma staging results of surgical removal of the hemangioma specimens in 49 cases, 27 cases in the proliferative phase, in the degradation of the 22 cases. 2 of cyclin D1 protein expression of cyclin D1 protein expression was located in the nucleus or cytoplasm of hemangiomas, brownish-yellow or brown granules, no expression in normal vascular endothelial cells. In the proliferative phase group, the average optical density and degradation of group and normal skin expression of cyclin D1 positive area ratio of 0.2903 ± 0.0068,0.2775 ± 0.0086 and 0.2678 ± 0.0011; 0.4448 ± 0.0918,0.1409 ± 0.0934 and 0.0862 ± 0.0570. Proliferative phase group and degradation of group, normal skin were compared, differences in cyclin D1 protein expression was significant (P lt; 0.05); degradation of group and normal skin between cyclin D1 protein expression differences no significant (P gt; 0.05) P27 protein expression of p27 protein expression was located in the nucleus or cytoplasm of hemangiomas, brownish yellow or brown granules abundantly expressed in normal vascular endothelial cells. In the proliferative phase group, degradation of group

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