Dissertation > Excellent graduate degree dissertation topics show

The Study on the Relationship between h1 Calponin mRNA Expression in Biliary Tract and Gallstone Formation in Pregnancy

Author: WangBin
Tutor: DingYouMing
School: Wuhan University
Course: Surgery
Keywords: Gene expression Contraction protein Smooth muscle Gallstone Pregnancy
CLC: R714.2
Type: Master's thesis
Year: 2005
Downloads: 47
Quote: 1
Read: Download Dissertation

Abstract


Objiective To explore the effects of h1 calponin mRNA expression on the biliary tract dynamics and investigate the molecular mechanisms of gallstone formation in pregnancy.Methods 30 femal guinea pigs were divided randomly into 3 groups, the nonpregnant group（n=10）, the 30 days of pregnancy（n=10） and the 60 days of pregnancy group（n=10）. Animal models of pregnancy were established on experimental guinea pigs through feeding animals with one cage according to female versus male as 4: 1. The bile, the portal vein blood, the gallbladder smooth muscles and Oddi sphincters were obtained respectively . The concentration of total cholesterol （TC）, total bilirubin （TBiL） and total bile acid （TBA） in the bile, the serum estradiol（E2） and progesterone（Pg） levels were determined respectively. Expression levels of hi.calponin mRNA in gallbladder smooth muscles and Oddi sphincters（OS） were evaluated by using semiquantitative reverse tanscription polymerase chain reaction（RT-PCR）.Results The levels of TC, TBiL and the serum E2 and Pg were significantly increased in pregnant guinea pigs, however, that of TBA decreased. Up-regulation of hi.calponin gene expression was observed in gallbladder in the 60 days of pregnancy, but converse in Oddi sphincters. The difference between the nonpregnant group and the 60 days of pregnancy group was remarkable.Conclusion Up-regulation of hi.calponin gene expression in gallbladder smooth muscles and down-regulation in Oddi sphincters might play a key role in induced dysfunction of extrahepatic biliary tract, bile stasis and gallstone formation in pregnancy.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. The Expression Survey of BMP Signalling Pathway in the Human Embryonic Tooth Germ,R78
  3. Study on Synthesis and Physiological Function of N-carbamoyl-glutamate,R914
  4. Functional Analysis of Two Rice Nitrate Transporter Genes OsNRT1.1a and OsNRT1.1b,S511
  5. Coparative Analyses of Carbon and Nitrogen Metabolism of Flue-cured Tobacco Growing in Henan and Yunnan,S572
  6. Study of Chicken Δ~6 Fatty Acid Desaturase Gene’s Promotor Region Polymorphism and Gene Expression,S831
  7. The Expression of CPV-2 VP2 Gene in SF9 and Development of Indirect Elisa for Serum Antibodys,S852.65
  8. Comparison of Gene Expression Data Cluster Methods and Gene Network Construction for Phytophthora Sojae Genes,S435.651
  9. Functional Identification of Two Tomoto Phosphater Transporter Genes LePT1 and LePT2 in Rice,S511
  10. Analysis for Intron 1 and 2 of Rice Transformation to Promoter Activity,S511
  11. Research on Characteristics of Growth, Development and Material Metabolism of Flue Cured Tobacco Leaves in Huili, Sichuan,S572
  12. Studies on Extracting Mitochondrial DNA and Gene Expressions in Brassica Napus L,S565.4
  13. Functional Characterization of Wheat Na~+ /H~+ Antiporter TaNHX2 and Study of TaNHX2 C- Terminus Domain,S512.1
  14. Identification and Analysis of miRNA and Flower Development Specific Genes in Wheat,S512.1
  15. Molecular Characterization, Expression, and Promoter Methylation Status of the Bovine Synaptonemal Complex Protein 3 Gene,S823
  16. The Deposition Rule and Gene Expression Involved in Yolk Cholesterol in Different Layer Breeds,S831
  17. Study on the mRNA Expression Level and the Polymorphism of Follicle-Stimulating Hormone Receptor Gene in Taihu Pig’s Ovary,S828
  18. The mRNA Expression of PAPPA, PLAC8 and PAG in Pregnant Diary Cows,S858.23
  19. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  20. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  21. The Design and Implementation of Bicluster Data Analyzing Software,TP311.52

CLC: > Medicine, health > Obstetrics and Gynaecology > Obstetrics > Pathological pregnancy ( abnormal pregnancy )
© 2012 www.DissertationTopic.Net  Mobile