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Virus-free Plantlet Culture and in Vitro Bulbil Induction of Dioscorea Batatas Decne.

Author: YangZuo
Tutor: YangBaiYun
School: Nanchang University
Course: Botany
Keywords: Foot potato Detoxification Bulbils Dissection Physiological and biochemical
CLC: S567.239
Type: Master's thesis
Year: 2006
Downloads: 79
Quote: 0
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Abstract

To Jiangxi, the test on the high-yielding potato's feet for the material, the use of plant tissue culture and technology paraffin sections, respectively feet potato virus-free seedling cultivation, detoxification foot plate potato foundation seedling propagation, the test tube bulbil induction and induction process bulbils development anatomy and physiological and biochemical changes. Provide a theoretical basis and key technologies for industrial production of virus-free the baseboard potato test tube bulbils. Feet potato no vaccine for the material, combined with shoot tip culture Foot potato detoxification effect of high temperature and virus chemical inhibitor pretreatment. The results show that: Foot potato shoot tip size and survival was positively related to detoxification rate was negatively correlated; a single detoxification treatment technology is difficult to completely remove the virus, potato Y 2 or 3 kinds of combination use of the technology can be effectively removed virus (PVY), tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV), compared with the control group, high temperature and ribavirin combination of high temperature pre-treatment, the detoxification rate increased by 20% and 40%. Virus-free seedlings stem segments cultured feet potato the foundation seedling propagation system. The results show that: the the internode proliferation culture medium for MS NAA 0.8 mg / L 6-BA 0.8 mg / L; induced stem segments to form multiple shoots best medium for MS NAA 0.5 mg / L 6-BA 4.0 mg / L uniconazole 3.0 mg / L, 4.0% sucrose. The solid-liquid double training methods to study the impact of different factors on detoxification foot plate the potato test tube beads bud induction. The results show that: the sucrose concentration of 8% light 8h / d of test tube bulbil formation and enlargement better: glutamine as the nitrogen source, have a significant role in promoting the the test tube beads bud enlargement; certain ratio of 6-BA and NAA favor bulbils induction, in which 6-BA 2.0 mg / L NAA 0.5 mg / L as well. The detoxification foot plate the potato test tube bulbil is occurred in the leaf axils, under the epidermis, three cell dedifferentiation and split to form bulbils primordium further develop into bulbils. Meristem below the top of the bulbils a newborn thickening meristem cell division and its derivative cell volume increases is early bulbil Expanding the main reason. With the growth and development of the bulbils, the primary thickening meristem derived secondary thickening meristem. The mature bulbils swelling secondary thickening meristem cell division and cell volume increases. Mature bulbils storeyed cork, secondary thickening meristem tissue, was born in one of the basic organization and scattered limited outside the tough vascular bundle, starch and protein accumulation in the parenchyma cells of the vascular bundles around. Bulbils starch content in the test tube beads bud induction process has been showed an upward trend; total soluble sugars, sucrose content and POD activity with the beads buds of growth and development was parabola, to 60d, reached the highest value, and then decreased gradually; soluble protein content is presented liters - lowering - liters trend.

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