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In this thesis, the changes of the protein content of corn stalks apoplast during storage and Penicilllum expansum synergy between cellulase from a whole new perspective to explore the plant cell wall components of cellulase to factor. And preliminary study of the apoplast protein extraction process . With the extension of storage time , can be extracted from corn stalks apoplast protein dwindling , 34.71% of the protein content of only 6 months when fresh , after protein degradation or inactivation slowed , protein content stabilized. Stored corn stover apoplast protein endogenous cellulase activity , while the activity of endogenous EG fresh corn stalks , but it is less stable store six months after the to basic degradation or inactivation . Between the obvious synergy between the apoplast protein P.expansum cellulase synergy relationship weakened with prolonged storage time . , Storage straw Apoplast protein When the the apoplast protein concentration in the reaction system is approximately 1mg/ml PFA viability , the cotton enzyme activity , β - glucosidase activity maximum synergistic effect , the increased efficiency of 95.32% , respectively , 102.06 % and 96.6 %, has shown inhibition of CMCase maximum inhibition rate was 49.52% . From the apoplast protein P.expansum cellulase components of the relationship between the point of view , this synergistic relationship , mainly apoplastic protein -EG-BG synergies , while in the cellulose hydrolysis process the CBH enzyme activities play a central role is not affected. To eliminate the influence of endogenous EG , fresh corn stalks apoplastic proteins PFA vitality , cotton enzyme , β - glucosidase enzyme to promote rate , as well as CMCase inhibition rate is higher than the quality of the storage straw
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