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Tetramethylpyrazine extraction and purification and stability studies
Author: XuWeiQuan
Tutor: MaRunYu
School: Beijing University of Chemical Technology
Course: Biochemical Engineering
Keywords: Tetramethylpyrazine Macroporous resin Separation and purification Oxidation resistance
CLC: TQ461
Type: Master's thesis
Year: 2005
Downloads: 372
Quote: 1
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Abstract
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Chuanxiong as Umbelliferae Chuanxiong of Ligusticum chuanxiong of Hort., Roots. Pharmacological experiments show Ligustrazine Injection clinical as cardiovascular drugs, increase coronary blood flow, improve the viscosity of whole blood and red blood cell deformability, suppression the thromboxane A 2 (TX A 2 sub >) biosynthesis, reduce ischemic brain tissue damage and nervous system dysfunction, and some lung and kidney disease therapies. Tetramethylpyrazine (Ligustrazine) is an active alkaloid extracted from the Chinese medicine Chuanxiong (Ligusticum chuanxiong Hort) - tetramethyl pyrazine. Pharmacological test showed that ligustrazine injection clinically as a cardiovascular drug, increase coronary blood flow, improve the whole blood viscosity trace red blood cell deformability, inhibiting thromboxane A2 (TXA2) biosynthesis, reduce ischemic brain damage and neurological dysfunction and certain lung, kidney disease therapy. Macroporous resin (macroporous absorption resin) is a functional polymer materials by polymerization of a monomer and crosslinker, porogen dispersion agent additives prepared from the polymerization reaction. Its physical and chemical properties of stable, insoluble in acids, alkalis and organic solvents, can selectively adsorb organic substances from aqueous solution by physical adsorption. The subject test screening of a large hole - the effect of comparing various types of resin separation and purification of tetramethylpyrazine standard solution, AB-8 resin has been with the best adsorption separation. The extraction and purification of TMP, to optimize the extraction conditions, the macroporous conditions, experimentally investigated the factors that affect the AB-8 resin adsorption of tetramethylpyrazine extract. Includes sample loading (leaching Chuanxiong quality / pillars resin quality), resin adsorption time, eluent flow rate, eluent concentration, the elution times and the resin is regenerated after the adsorption effect. Draw the best purification effect of each factor to determine the optimum extraction conditions; ligustrazine hydrochloride stability and improve the stability of an effective method to study the temperature, add the type and quantity of antioxidants of ligustrazine hydrochloride stability. Arrive at the same mass concentration antioxidant, vitamin C, better than citric acid. Inhibition and antioxidant activity of vitamin C for a certain concentration of citric be.
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