Dissertation > Excellent graduate degree dissertation topics show

Electrochemical Studies on the New Systems of 3,4-Dianimobenzoic Acid-H2O2-Horseradish Peroxidase （HRP） Voltammetric Enzyme-Linked Immunoassay and Their Applications in Immunoassay

Author: DuPing
Tutor: ZhangShuSheng
School: Qingdao University of Science and Technology
Course: Applied Chemistry
Keywords: 3,4-Dianimobenzoic acid Horseradish peroxidase Voltammetric Enzyme-catalysis Enzyme- linked immunoassay
CLC: R446.6
Type: Master's thesis
Year: 2006
Downloads: 41
Quote: 1
Read: Download Dissertation

Abstract


Immunoassay with electrochemical detection is an attractive method due to its high sensitivity and low detection limit. It can also compensate its deficiency in differentiation with immunity. So it is of interest because of wide detection range and low detection limit. It is suitable for the analysis of small amounts, the instruments are simple, and the operation is convenient. So the linear sweep voltammetric was used to study the 3,4-Dianimobenzoic acid-H2O2-horseradish peroxidase （HRP） voltammetric enzyme-linked immunoassay new system. So far, the systems were not repoted by others. The optimal condition of enzyme-catalyzed reaction were investigated, and the best reaction condition of these systems were selected. In addition, the virus clinico detection was also performed. The 3,4-Dianimobenzoic acid-H2O2-horseradish peroxidase （HRP） voltammetric enzyme-linked immunoassay new system had extremely high sensitivity. HRP can be measured with a detection limit of 6.0×10-13 g/mL and a linear range of 4.0×10-9 4.0×10-12 g/mL.This new voltmmetric enzyme-linked immunossy systerm has firstly been successfully applied to the detection of αFP. The linear range is 0.20-50 ng/mL. Under the optimum conditions, the human serum samples were detected. The results showed good corresponding relationship with those in spectrophotometric ELAISA method.This new voltmmetric enzyme-linked immunossy systerm has firstly been successfully applied to the detection of CEA. The linear range is 0.50-80 ng/mL.Under the optimum conditions,the human serum samples are detected.The results have good corresponding relationship with those in spectrophotometric ELIS A method.

Related Dissertations

  1. The Preparation and Its Aplications of Polyaniline Chemically Modified Electrodes,O657.1
  2. Rabbit neck lamina dura adjacent surfaces and experimental studies innervation,R681.5
  3. Detemination of Folic Acid on PtNPs/MWNTs Modified Glassy Carbon Electrode,O657.1
  4. Fabrications and Applications of Microelectrode Array of Silicon Dioxide Cavities and Magnetic Ferroferric Oxide Nano-Bioconjunctions,TB383.1
  5. Nano-ZnO preparation, characterization, and its modified electrode electrocatalytic biomolecules,O614.241
  6. The Study on the Nervus Gastrica Domination of Rana by Using House Radish Peroxidase Method,S917.4
  7. Preparation and Characterization of P(DEA-co-AA) Microgels and Its Role in Horseradish Peroxidase Immobilization,O631.3
  8. Inorganic / bio- organic nano- hybrid membrane electrode based sensing , inhibition kinetics of research and its application,TS207.3
  9. Phase I Clinical Pharmacokinetic Study of Anti-recombinant Human Cytotoxic T Lymphocyte Related Antigen-4 in Rheumatoid Arthritis Patients,R593.22
  10. And its analytical determination of singlet oxygen in samples of biological drugs,R917
  11. Water molecules on the catalytic decarboxylation of orotic acid - nucleoside monophosphate,O621.2
  12. Study on Inactivation Kinetics of POD & PG in Pulsed Electric Fields,TS201.1
  13. Non-aqueous micellar nature and micellar enzyme catalyzed calorimetry,O648.1
  14. Fabrication of Two-dimensional Nano-gold Particles Monolayer and Its Application,TB383.1
  15. Application of Potentiometric Multienzyme Biosensors in the Determination of Organphosphorus Pesticides,X839.2
  16. Enzyme-Catalyzed Enantioselective Hydrolysis of Ethyl Mandelate in Ionic Liquids,TQ463.2
  17. Preparation and Application of Pillared Clay Modified Electrode,O657.1
  18. Study and Application with Horseradish Peroxidase,Laccase Biosensores,TP212.3
  19. Bioelectrochemistry Studies Based on Functional Halloysite Nanomaterials,TP212.3
  20. Preliminary Experimental Studies of DNA Electrochemical Biosensor Modified by Gold Nanoparticles for Detection of PML/RARα Fusion Gene in Acute Promyelocytic Leukemia,R733.71

CLC: > Medicine, health > Clinical > Diagnostics > Laboratory diagnosis > Immunology Examination
© 2012 www.DissertationTopic.Net  Mobile