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The Circuit Design of Voltage-Clamp-Controlled Current-Clamp
Author: FanFeng
Tutor: ZuoAnLian
School: Huazhong University of Science and Technology
Course: Biophysics
Keywords: Patch-clamp amplifier Current Clamp Voltage -controlled current clamp clamp
CLC: Q-337
Type: Master's thesis
Year: 2005
Downloads: 101
Quote: 0
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Abstract
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The birth of the patch-clamp technique electrophysiological experiments technical step onto a new level , in the field of life sciences research has been widely applied quickly . With the patch-clamp technique itself is also electrophysiological studies of development and continue to progress. But as more and more people are using patch-clamp amplifier (PCA) in current clamp mode to record rapid changes in cell voltage signal , the researchers found recorded waveform amplitude and phase distortion will occur significantly . To be able to fundamentally improve the current clamp performance , first from the circuit theory and experimental data and other aspects of the recording waveform distortion occurs because the nature and the specific occurrence of the process , through the circuit transfer function analysis , theoretically PCA current clamp mode theory flaws make the system complete explanation . Radically improve the performance of current clamp only way is to redesign the circuit structure is more reasonable , including the PCA probe substantially altered. The new current clamp circuit reference voltage follower classic structure that can achieve a real sense of the current and voltage clamp recording , so as to optimize the performance of PCA current clamp mode purposes. Experiments show that the new circuit not only successfully enhance current clamp performance , but also to maintain the original voltage clamp function and noise performance. To meet the needs of certain electrophysiological experiments clamp current clamp steady-state voltage , while the actual needs of the new current clamp circuit structure based on the design of a new PCA mode - voltage clamp -controlled current clamp mode . Circuit Test results show designed to achieve the desired vision , have practical value.
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CLC: > Biological Sciences > Biological science research methods and techniques > Biological experiments and biological techniques > Laboratory equipment and devices
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